期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

阿托伐他汀对人肾小球系膜细胞增殖、TGF-β1 mRNA和p38MAPK表达的影响 被引量：2

EFFECT OF ATORVASTATIN ON CELL PROLIFERATION,TGF-β1 mRNA AND p38MAPK EXPRESSION
下载PDF
导出
摘要 目的观察阿托伐他汀(atorv)对氧化低密度脂蛋白(ox-LDL)诱导的人肾小球系膜细胞(HGMCs)增殖和转化生长因子β1(TGF-β1)mRNA及丝裂原活化蛋白激酶(p38MAPK)蛋白表达的影响。方法在体外培养HG-MCs,用MTT法检测细胞增殖,用半定量RT-PCR法检测细胞TGF-β1 mRNA表达,用Western blot法检测细胞p38MAPK蛋白合成。结果1.Ox-LDL(80μg/ml)刺激系膜细胞增殖;2.Ox-LDL(10μg/ml-80μg/ml)以浓度依赖的方式增加HGMCs TGF-β1 mRNA和p38MAPK蛋白表达,3.Atovastatin(6μg-12μg/ml)抑制系膜细胞增殖,降低ox-LDL引起的TGF-β1 mRNA表达上调,抑制p38MAPK信号途径激活。结论阿托伐他汀可能通过对抗p38MAPK信号通路,减少TGFβ1分泌,抑制ox-LDL引起的肾小球系膜细胞增殖,预防和治疗伴有血脂异常的糖尿病肾脏病变。 Objective 1. To observe the effect of atorvastatin on cell proliferation and expression of TGF-β1 mRNA and p38MAPK in human glomerular mesangial cells （HGMCs） induced by ox-LDL. Methods Cultured human glomerular mesangial cells were used. Cell proliferation was measured by MTT, TGF-β1 mRNA expression by RT-PCR, and p38MAPK protein expression in HGMCs by Western blot. Results 1. Ox-LDL （80μg/ml） stimulated mesangial cell proliferation; 2. Ox-LDL （10μg/ml-80μg/ ml） increased the expression of HGMCs TGF-β1 rnRNA in a concentration-dependent manner; 3. Atorvastatin （6μg/ml- 12μg/ml） inhibited mesangial cell proliferation and attenuated TGFβ1 mRNA and p38MAPK expression upregulation induced by Ox-LDL. Conclusion Atorvastatin may exert its beneficial effect by inhibiting mesangial cell proliferation and attenuating TGF-β1 mRNA and p38MAPK expressions in diabetic nephropathy.
作者 李萍 张海燕 刘国良 盛志业 马小峰 向军
机构地区 沈阳军区总医院内分泌科 中国医科大学附属第一医院内分泌科 沈阳军区总医院呼吸内科 沈阳军区总医院泌尿外科
出处 《中国组织化学与细胞化学杂志》 CAS CSCD 2007年第1期57-61,共5页 Chinese Journal of Histochemistry and Cytochemistry
基金 辽宁省重点科技攻关项目(2001225004)
关键词 阿托伐他汀 细胞增殖 转化生长因子Β1 丝裂原活化蛋白激酶 人肾小球系膜细胞 Atorvastatin Cell proliferation Transforming growth factor β1 p38 mito-gen-activated protein kinases Human glomerular mesangial cell
分类号 R587.1 [医药卫生—内分泌]
  • 相关文献

参考文献18

  • 1Striker LJ,Dio T,Elliot S,et al.The contribution of glomerular mesangial cells to progressive glomeruloslersis.Semin Nephrol,1989,9:318-328
  • 2Eng E,Floege J,Young BA,et al.Does extracellular matrix expansion in glomerular disease require mesangial cell proliferation? Kidney Int,1994,Suppl 45:S45-S47
  • 3Ujihara N,Sakka Y,Takeda M,et al.Association between plasma oxidized low-density lipoprotein and diabetic nephropathy.Diabetes Res Clin Pract,2002,58:109-114
  • 4Wolfrum S,Dendorfer A,Schutt M,et al.Simvastatin acutely reduces myocardial reperfusion injury in vivo by activating the phosphatidylinositide 3-kinase/Akt pathway.J Cardiovasc Pharmacol,2004,44(3):348-355
  • 5Gotz AK,Boger CA,Hirschmann C,et al.Effect of HMG-CoA-reductase inhibitors on survival in type 2 diabetes patients with end stage diabetic nephropathy.Eur J Med Res,2005,10 (4):155-160
  • 6Paolo Mene.Mesangial cell cultures.J Nephro,2001,14 (3):198-203
  • 7Usui H,Shikata K,Matsuda M,et al.HMG-CoA reductase inhibitor ameliorates diabetic nephropathy by its pleiotropic effects in rats.Nenhrol Dial Transplant,2003,18(2):265-272
  • 8Nogaki F,Muso E,Yashiro,et al.Direct inhibitory effects of simvastatin on matrix accumulation in cultured murine mesangial cells.Kidney Intd,1999,71(suppl):198-201
  • 9Fukada Y,Takao T,Ohgura H,et al.Farnesylated gamma-subunit of photoreceptor G protein content indispensable for GTP-binding.Nature,1990.346:958-660
  • 10Vrtovsnik F,Courtte S,Prie D,et al.Lovastatin-induced inhibition of renal epithelial tubular cell proliferation involve a p21ras activated AP-1dependent pathway.Kindney Int,1997,52:1016-1027

二级参考文献39

  • 1王淳本,宗义强,吴万生,冯友梅,邓耀祖,冯宗忱.两步超速离心法快速分离大量血浆极低密度脂蛋白及低密度脂蛋白[J].同济医科大学学报,1995,24(3):169-171. 被引量：92
  • 2David AC, Robina C. Molecules in focus: transforming growth factor-beta (TGF-β). Int J Biochem Cell Biol, 1998, 30: 293-298.
  • 3Cora W, Katrin B, Hans UH, et al.Low-molecular-weight heparin prevents high glucose- and phorbol ester-induced TGF-β1 gene activation.Kidney Int, 2001, 60: 935-943.
  • 4Ding GH, Harry VG, Sharon D, et al. Oxidized LDL stimulates the expression of TGF-β and fibronectin in human glomerular epithelial cells. Kidney Int, 1997, 51:147-154.
  • 5Maziere C, Mergny MD, Fressart VF, et al.Copper and cell-oxidized low-density lipoprotein induces activator protein-1 in fibroblasts, endothelial and smooth muscle cells.FEBS Letters, 1997, 409:351-356.
  • 6Yang CM, Chiu CT, Wang CC, et al. Activation of mitogen-activated protein kinase by oxidized low density lipoprotein in canine cultured vascular smooth muscle cells. Cell Signal, 2000,12:205-214.
  • 7Wu ZL, Liang MY, Qiu LQ. Oxidized low density lipoprotein decreases the induced nitric oxide synthesis in rat mesangial cells. Cell Biochem Funct, 1998,16:153-155.
  • 8Andrews NC, Faller DV.A rapid micropreparation techique for extraction of DNA-binding proteins from limiting numbers of mammalian cells.Nucleic Acids Res, 1991,19: 2499-2501.
  • 9Joseph S, Russell DW. Molecular cloning: a laboratory manual. 3rd ed. New York: Cold Spring Harbor Laboratory Press, 2001, 17.42.
  • 10Shi XL, Ding M, Dong ZG, et al. Antioxidant properties of aspirin: characterization of the ability of aspirin to inhibit silica-induced lipid peroxidation, DNA damage, NF-κB activation, and TNF-α production. Mol Cell Biochem, 1999, 199:93-102.

共引文献21

同被引文献35

  • 1ZequanJi,CuiwenHuang,ChengjieLiang,BoChen,ShengqiangChen,WeiwenSun.Protective Effects of Blocking Renin-Angiotensin System on the Progression of Renal Injury in Glomerulosclerosis[J].Cellular & Molecular Immunology,2005,2(2):150-154. 被引量：11
  • 2张贺,韩子明,杨达胜.洛伐他汀对肾小球硬化大鼠纤溶酶原激活物抑制剂-1和Ⅳ型胶原表达的影响[J].实用儿科临床杂志,2007,22(5):338-339. 被引量：4
  • 3CANNON C P, BRAUNWALD E, MCCABE C H, et al. Pravastain or atorvastatin evaluation and infectioth therapy-thrombolysis in myocardial infarction 22 investigators. Intersive versus moderate lipid lowering with statin ofter acute coronary syndromes[J]. N Engl J Med, 2004,350: 1495-1504.
  • 4TONOLO G, MELIS G M, FORMATO M, et al. Additive effects of simvastatin beyond its effects on LDL cholesterol in hypertensive type 2 diabetic patients [J] Eur J Clin Invest, 2000,30:980-987.
  • 5Wolf G.Cell cycle regulation in diabetic nephrepathy[J].Kidney Int,2000,58 (Suppl 77):S59-66.
  • 6Buranakarl C,Kitjtawonrat A,Pondeenana S,et al.Comparison of dipyridamole and fosinop ril on renal progreesion in nephrectomized rats[J].Nephrology (Carlton),2003,8 (2):80-91.
  • 7Ziyadeh FN.Mediators of diabetic renal disease:the case for TGF-βas the major mediator[J].J Am Soc Nephrol,2004,15 (Suppl 1):S55-57.
  • 8Dessapt C,Baradez MO,Hayward A,et al.Mechanical forces and TGF { beta } 1 reduce podocyte adhesion through {alpha} 3 { beta} 1 integrin downregulation[J].Nephrol Dial Transplant,2009,24 (9):2645-2655.
  • 9Lee EY,Chung CH,Khoury CC,et al.The monocyte chemoattractant protein-1/CCR2 loop,inducible by TGF-beta,increases podocyte motility and albumin permeability[J].Am J Physiol Renal Physiol,2009,297 (1):F85-94.
  • 10Tuttle KR.Linking metabolism and immunology:diabetic nephropathy is an inflammatory dissese[J].J Am Soc Nephrol,2005,16 (6):1537 -1538.

引证文献2

二级引证文献6

中国组织化学与细胞化学杂志
2007年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部