反复热性惊厥过程中气体信号分子硫化氢对一氧化氮/一氧化氮合酶体系的影响

Hydrogen sulfide regulates the expression of nitric oxide/nitric oxide synthase system during recurrent febrile seizures

目的:探讨硫化氢(H2S)对热性惊厥(FS)大鼠一氧化氮(NO)/一氧化氮合酶(NOS)体系表达的影响。方法:大鼠随机分为对照组、FS组、FS+NaHS组、FS+HA(hydroxylam ine)组。采用热水浴诱导大鼠FS,隔日诱导1次,共10次。采用分光光度计法测定大鼠血浆中H2S和NO含量;用原位杂交观察nNOS mRNA表达情况;用免疫组化方法观察NOS蛋白表达情况。结果:FS+NaHS组NO含量低于FS组,同时NOS表达也低于FS组;而FS+HA组NO含量高于FS组,同时NOS表达也强于FS组。结论:用H2S外源性供体NaHS和胱硫醚-β-合成酶抑制剂HA的干预研究表明,反复热性惊厥过程中,H2S的改变可影响NO/NOS体系的表达。

AIM： To explore the effect of hydrogen sulfide （H2S） on nitric oxide （NO）/nitric oxide synthase （NOS） system during recurrent febrile seizures （FS）. METHODS： Sprague - Dawley rats aged 21 days were randomly divided into four groups ： control group （ 37.0 ℃ water, n = 8 ） ; FS group （ 45.2 ℃ water, n = 8 ） ; FS ＋ NaHs group （45. 2 ℃ water, n =8）, FS ＋ HA （hydroxylamine） group （45.2 ℃ water, n =8）. FS in rats were induced ten times in a bath of warm water, once every 2 days. The plasma level of H2S and NO was detected by the spectrophotometer method. The expression of NOS mRNA was examined by in situ hybridization. The expression of nNOS protein was observed by immunohistochemistry. RESULTS ： The plasma level of NO decreased significantly in FS ＋ NaHs group while elevated obviously in FS ＋ HA group compared with that in FS group. At the same time, the expression of nNOS down - regulated in FS ＋ NaHs group while up - regulated in FS ＋ HA group compared with that in FS group. CONCLUSION： H2S down - regulated the expression of NO/NOS system during recurrent FS.