兔眼结膜上皮细胞体外培养治疗结膜缺损实验研究

The Cultured Human Conjunctival Epithelium Combined with Amniotic Membrane Transplantation for Treatment of Conjunctival Defect

目的探讨体外分离培养结膜上皮细胞治疗结膜缺损的方法。方法新西兰大白兔10只,右眼(10眼)为实验组,左眼(10眼)为对照组。术前2周取右眼穹隆部结膜1mm×1mm剪成0.5mm×0.5mm后种植于羊膜上体外培养;术中切除鼻侧结膜组织,实验组将结膜上皮植片移植于结膜创口,对照组结膜创口暴露。观察结膜上皮细胞的生长特性。结果接种在羊膜上的结膜组织,6天左右融合成膜状,细胞为复层上皮细胞。术后实验组角膜透明,结膜移植片与周围组织完全融合,未发生排斥反应;对照组为瘢痕性愈合。结论体外分离培养结膜上皮细胞是治疗结膜缺损的一种理想的好方法。

Objective To study the method of the cultured human conjunctival epithelium combined with amniotic membrane transplantation for treatment of conjunctival defect. Methods 20 eyes of 10 rabbits were divided into study group （ 10 right eyes ） and contoal group （ 10 left eyes ）. The conjunctival epithelium got from conjunctiva of fornix of study group about 1 mm × 1 mm was explanted on hu- man amniotic membrane and was cultured in vitro by tissue inoculation 2 weeks before operation. The bulbar conjunctivals of 20 eyes were excised. The conjunctival defect was covered by the graft in the study group and uncovered in the control group. The growth features of the grafts were observed. Results The conjunetival epithelium explanted on human amniotic membrane connected each other into membrane in 6 days, and multi - layer cells were observed. In the study group, all grafts were inosculated with and were similar to normal conjunctival in 2 weeks. There was not immune rejection occurred. In the control group, the conjunctival defect was recovered by scar. Conclusion It is effective of the cultured conjunctival epithelium combined with amniotic membrane transplantation for treatment of conjunctival defect.