鱼腥草挥发油在家兔体内的药动学研究

Pharmacokinetic study on volatile oil from whole herbs of Houttuynia cordatain rabbits in vivo

目的以甲基正壬酮为指标，研究鱼腥草挥发油在家兔体内药动学参数，确定其药动学模型。方法使用气相色谱法检测鱼腥草挥发油中甲基正壬酮在家兔血浆中的血药浓度，采用SPB-5毛细管气相色谱柱，程序升温：起始温度90℃，以10℃／min升至190℃，保持5min，进样口温度250℃，FID检测温度300℃，进样量5．0μL，无分流进样，溶剂为正己烷；载气为氮气，柱头压55.2kPa，内标物正十五烷；并运用3P97程序对甲基正壬酮的血药浓度测定值进行拟合。结果建立了用气相色谱法测定鱼腥草挥发油中甲基正壬酮血药浓度的方法，甲基正壬酮在0．04～60．0μg／mL线性关系良好（r=0．987O）；其药-时过程符合二室开放模型。结论本实验为鱼腥草药材的合理用药及二次开发提供了药动学依据；同时也为中药材挥发油类成分的药动学研究提供了可借答的方法．

Objective Taking methyl nonyl ketone as index to establish pharmacokinetic compartment model for study volatile oil from the whole herb of Houttuynia cordata by pharmacokinetic parameters in rabbits in vivo. Methods Using GC to determine the blood concentrations of methyl nonyl ketone in the volatile oil from whole herbs of tt. cordata in rabbit plasma. The GC system used a capillary SPB-5 column and helium as carrier gas; the initial temperature was at 90 ℃, then raised to 190 ℃ by 10℃/min and kept for 5 min; the injector temperature was 250 ℃ and the FID detector temperature was 300 ℃ ; the injection volume was 5.0 μL with splitless injection mode; n-hexane was used as solvent and n-pentadecane as interior standard substance. The blood concentration value of methyl nonyl ketone was fitted with 3P97 procedure. Results A capillary GC method was established for the detemination of methyl nonyl ketone in the volatile oil from whole herbs of H. cordata in rabbit plasma. Methyl nonyl ketone linearity was good （r=0. 987 0） within 0.04-60. 0 μg/mL. The blood concentration-time course of precision in rabbit plasma conformed to two compartment model. Conclusion The experiment provides pharmacokinetic evidence for the rational administration and the further development of H. cordata and some methods for pharmacokinetic study of volatile oils from traditional Chinese medicinal materials.