杜仲药材HPLC指纹图谱及其共有模式的建立

Establishment of fingerprint of Cortex Eucommiae and its mutual mode by HPLC

目的用高效液相色谱法建立杜仲药材的指纹图谱分析方法。方法采用Lichrospher C18（250mm×4．6mm，5μm）分析柱，甲醇-0．1％磷酸水为流动相，梯度洗脱，体积流量为1．0mL／min，检测波长为230nm，柱温30℃。结果共有11个共有峰，采用《中药色谱指纹图谱相似度评价系统（2004A版）》，计算各被测样品的HPLC指纹图谱的整体相似度，10批被测样品的色谱指纹图谱的整体相似度在0．5740．958，并得出了杜仲药材的对照指纹图谱。结论该指纹图谱方法简便、重现性好，可专属性地研究杜仲药材的指纹图谱，为有效地控制杜仲药材的内在质量提供了科学依据。

Objective To establish the fingerprints for analysis of Cortex Eucommiae by HPLC. Methods The column of Lichroshper C18 （250 mm × 4. 6 mm, 5 μm） was used. The mobile phase consisted of methanol-0. 1 % phosphoric acid with gradient elution. The detective wavelength was 230 nm, the column temperature was 30 ℃, and the flow rate was 1.0 mL/min. Results The fingerprint consisted of 11 common peaks. The mutual mode of HPLC fingerprints was set up and the similar degrees to the crude drugs from different habitats were compared by Similarity Evaluation System for Chromatographic Fingerprint of CMM （Version 2004A）, and the range of similarity for ten balches of Cortex Eucommiae 0. 574-0. 958. The standard HPLC fingerprint of Cortex Eucommiae was established too. Conclusion This method is accurate and reliable, and provides a scientific basis for the quality control of Cortex Eucommiae.