白细胞介素-2对高糖状态下人视网膜色素上皮细胞的影响

The incpact of interleukin-2 on human retinal pigment epithelial cells in high glucose

目的研究白细胞介素-2（IL-2）对高糖状态下人视网膜色素上皮（RPE）细胞的增殖及其分泌和表达血管内皮细胞生长因子（vascular endothelial growth factor，VEGF）的影响。方法MTT自动比色法观察IL-2浓度对高糖状态下RPE细胞增殖的作用；ELISA法测RPE细胞分泌VEGF的变化；免疫组化观察RPE细胞表达VEGF的变化。结果0．1～10μg／L的IL-2均能明显促进高糖状态下RPE细胞的增殖，并能明显提高RPE细胞分泌VEGF的水平及提高VEGF在细胞中的表达。结论高糖状态下，IL-2可明显促进人RPE细胞的增殖并能提高RPE细胞分泌VEGF的水平和提高VEGF在RPE细胞中的表达，IL-2可能在增殖性糖尿病视网膜病变中起一定的作用。

Objective To study the effects of interleukin（IL）-2 on the proliferation of cultured human retinal pigment epithelial（CHRPE） cells in high glucose, vascular endothelial growth factor （VEGF） level and expression in CHRPE cells in high glucose. Methods The proliferation of CHRPE cells was determined with MTr method and the level of VEGF in culture medium or the expression of VEGF in RPE cells was measured by ELISA or immunohistochemistry. Results IL-2（0.1～10μg/L） obviously stimulated the proliferation of CHRPE cells in dose-dependent manner in high glucose and the VEGF concentration was increased significantly in culture medium of CHRPE cells. The expression of VEGF in RPE cells was also increased significantly. Conclusion In high glucose culture medium, IL-2 can stimulate the proliferation and increase VEGF level in culture medium ,as well as upregulate the expression of VEGF in CHRPE cells. IL-2 maybe play a role in proliferative diabetic retinopathy.