电针对脑缺血再灌注损伤小鼠海马神经元凋亡及Bcl-2、Bax表达的影响

Effects of Electropuncture on Apoptosis and Expressions of Bcl-2 and Bax in Hippocampus Neurons of Cerebral Ischemia Reperfusion Injury Mice

目的:观察电针对脑缺血再灌注损伤小鼠海马神经元凋亡及相关基因Bcl-2、Bax表达的影响。方法:复制脑缺血再灌注小鼠模型,电针肾俞、膈俞和百会穴,对照组灌胃喜得镇,分别于治疗第1天和第7天后,以流式细胞术(FCM)法检测小鼠海马细胞凋亡率、Bcl-2、Bax表达。结果:术后第1天,模型小鼠海马细胞凋亡率明显增高,术后第7天较术后第1天增高(P<0.01)。说明海马细胞由于反复脑缺血再灌注而出现凋亡。模型组术后第1天,海马Bcl-2表达明显下降;术后第7天,则明显增高,并高于术后第1天(P<0.01)。模型组术后第1天和第7天海马Bax表达均增高(P<0.01)。电针在两个时点均可上调海马Bcl-2表达,下调Bax表达,降低凋亡率(P<0.01)。结论:电针具有抑制细胞凋亡、保护神经元的作用。

Objective： To explore the effects of electropuncture on apoptosis and expressions of Bcl-2 and Bax in hippocampus neurons of cerebral ischemia reperfusion injury mice. Methods： The models of mice were established by repeated cerebral ischemia-reperfusion, and were given electric acupuncture with points of Shenshu, Geshu, and Baihui. The mice of control group were administered intragastrically with hydergine. With method of flow cytometry, the apoptosis rate and expressions of Bcl-2 and Bax in hippocampus neurons of mice on the first and seventh day after treatment were detected. Results： The apoptosis rate of hippocampus cells increased obviously on the first and seventh day after operation （ P 〈 0.01 ）, which illustrated that hippocampus cells appeared apoptosis due to repeated cerebral ischemia-reperfusion. The expressions of Bcl-2 in hippocampus neurons decreased on the first day, but increased notable on the seventh day （ P 〈 0.01 ）. The expressions of Bax in hippocampus neurons increased notable on the first and the seventh day （ P 〈 0.01）. Electropuncture could up-regulate the expressions of Bcl-2, lower the expressions of Bax and lower the apoptosis rate of hippocampus cells of mice （ P 〈 0.01 ）. Conclusion： The treatment with electropuncture could inhibit apoptosis and protect neurons.