高效液相色谱法测定麦芽糖基-β-环糊精的含量

High Performance Liquid Chromatograph for the Determination of Maltosyl-β-cyclodextrin

研究了采用高效液相色谱法测定麦芽糖基-β-环糊精。通过Mark-NH2柱分离，流动相为40％乙腈水溶液，流速为1．0ml／min，柱温为30℃。回收率为99．49％-99．85％，变异系数（CV）为1．23％。

A method for measurement of maltosyl-β-cyclodextrin by high performance liquid chromatograph （HPLC） is described, maltosyl-β-cyclodextrin is separated by Mark-NH2 column, using acetonitrile-water （40：60, v/v） as the solvent system at the flow rate of 1.0ml/min （column temperature 30℃）.Its recovery is from 99.49 to 99.85 %. Coefficient of variation is 1.23%.