转录因子E2F-1在梗阻性肾组织中的表达及其意义

Expression of transcription factor E2F-1 in chronic obstructive kidneys

目的探讨转录因子E2F-1在梗阻性肾病的发病机制中作用。方法应用免疫组化方法检测E2F-1和增殖细胞核抗原(PCNA)在43例梗阻性肾组织和12例正常肾组织中的表达。分析E2F-1表达与梗阻性肾积水和肾纤维化程度之间关系。结果正常肾组织中E2F-1阳性表达率为41.7%,显著低于梗阻肾组织的79.1%(P<0.05)。梗阻肾组织E2F-1表达与肾纤维化程度及PCNA指数密切相关(P<0.05),但与肾积水程度无显著相关性(P>0.05)。结论E2F-1异常表达参与梗阻性肾病的发病过程,尤其在肾纤维化过程中发挥重要作用。

[Objectives] To investigate the role of transcription factor E2F-1 in molercular pathogenesis of chronic obstructive kidney （COK）. [Methods] The immunohistochemistry methods was adopted to examine the expressions of E2F-1 and proliferation cell nuclear antigen （PCNA） in 43 cases with COK and 12 cases with normal renal tissues. The relationships between expression of E2F-1 and degree of hydronephrosis and renal fibrosis were evaluated. [Results] 41.7% of E2F-1 positive expression rate was found in normal renal tissues, which significantly lower than 79.1% in COK （P 〈0.05）. Expressions of E2F-1 in COK were close associated with degree of renal fibrosis and PCNA index （P 〈0,05）, while insignificantly associated with degree of hydronephrosis （P 〉0.05）. [ Conclusion] Abnormality of E2F-lexpression is involved in development and progression of COK, especially plays an important role in renal fibrosis.