人神经母细胞瘤SK-H-SN细胞株的多药耐药性及逆转研究

Multidrug Resistance and Reversion study on Human Neuroblastoma SK-H-SN Cell Line

本文对人神经母细胞瘤SK－H－SN细胞株的多药耐药性及其逆转进行实验，LSAB免疫组织化学定位显示整个SK－H－SN细胞膜表面以及细胞质内都有较强的耐药性P-170糖蛋白；MTT细胞毒性分析法证实，分别合用维拉帕米和双嘧啶胺醇前后，SK－H－SN细胞株细胞对抗肿瘤药物阿霉素、长春新碱的敏感性均有显著性差异（P＜0．01）。表明维拉帕米和双嘧啶胺醇对人神经母细胞瘤SK－H－SN细胞株的耐药性有逆转作用。

Experiment was done on human neuroblastoma SK-H-SN cell line about its multidrug resistance and reversion. with ABC immunohistochemical localization, both the cytoplasma and cell membrane showed abundant p-glycoprotein staining, MTT cytotoxic assay revealed that the sensitivities of SK-H-SN cells to doxoruicine or vincristine were significantly different according to the presence or absence of verapamil or dipyridamole(p＜0. 01), which indicate that the drug resistance of SKH-SN cells can be reversed by verapamil or dipyridamole.