HPLC法测定复方降压素片中利血平含量

Determination of reserpine in compound hypotensive tablet by HPLC

目的：建立HPLC法测定复方降压素片中利血平的含量。方法：采用C18（200mm×4．6mm，5μm）色谱柱，乙腈-水（41：59）用高氯酸调节pH至2．15±0．05为流动相，检测波长268nm。结果：利血平在3．84～15．36μg/ml浓度范围内呈良好线性关系（r=0．9996），平均回收率为99．9％，RSD为0．65％（n=9）。结论：本方法简便、准确、可靠。

OBJECT To establish a HPLC method for the determination of reserpine in compound hypotensive tablet. METHODS The chromatographic system consisted of C18 column and mobile phase of acetonitrilewatar （ adjusted pH to 2.15 _ 0.05 with perchloric acid） （41： 59）. The detecting wavelength was set at 268 nm. RESULTS There was a good linear range of reserpine between 3.84 - 15.36ug/ml（ r =0. 999.6）. The average recovery was 99.9% and RSD was 0.65% （n = 9）. CONCLUTION This method is simple, accurate and reliable.