膀胱肿瘤中基底膜蛋白的表达和意义

Expressions of basement membrane proteins in bladder cancer and its significance

目的:研究浅表性膀胱肿瘤组织中基底膜胶原蛋白Ⅳ(typeⅣcollagen,ColⅣ)、层黏连蛋白(laminin,LN)的表达,以探讨基底膜蛋白与浅表性膀胱肿瘤组织分化及预后的关系。方法:应用免疫组化链霉亲和素生物素过氧化物酶复合物(streptavidinbiotincomplex,SABC)法检测32例浅表性膀胱肿瘤石蜡标本切片的基底膜ColⅣ和LN的表达。结果:随着肿瘤分期、分级的增高,colIV和LN表达下降(P<0.05)。初发组随访观察3～24个月,13例复发。复发患者基底膜ColⅣ、LN含量明显低于无复发肿瘤患者(P<005)。结论:基底膜中ColⅣ、LN缺失程度与癌的分化程度、侵袭力及肿瘤的复发有关,ColⅣ和LN可作为浅表性膀胱肿瘤的术后预测复发的指标。

Objectlve：To study the expression of type Ⅳ collagen（Col Ⅳ） and laminin（LN） in the basement membrane of bladder cancer so as to explore the relationships between the basement membrane and the degree of differentiation and prognosis of bladder cancer. Methods： Thirty-two paraffin-embedded specimen of bladder caner were studied to identify the expressions of Col Ⅳ and LN with immunohistochemical SABC techniques. Result： It is shown that the poorer the tumor differentiation,the less expression of Col Ⅳ and LN. And the expressions of COl Ⅳ and LN between superficial tumor and invasive tumor were significantly different. There was a negative correlation between the expressions of Col Ⅳ and LN and the tumor differentiation （P〈0.05）. In a short term follow-up（3 -24 month） of primary bladder cancer, 13 recurred. A statistically significant difference of expressions of Col Ⅳ and LN could be observed between recurring and unrecurring superficial tumor in the basement membrane（P〈0.05）. Conclusion： The absences of expressions of Col Ⅳ and LN are related to the invasive capacity and recurrence of the tumor. The expression of Col Ⅳ and LN may be an index for the judgement of superficial bladder caner.