温州汉族群体3个Y-STR基因座的遗传多态性调查

Polymorphism research of three Y-DNA STR loci in Han population in Wenzhou

目的:获得温州汉族Y-DNA位点:DYS385、DYS392、DYS448的基因频率,并能应用这些资料进行人类遗传学研究和法医学应用。方法:以Chelex法提取外周血DNA,PCR扩增短串联重复(STR)位点DYS385、DYS392、DYS448后,用聚丙烯酰胺电泳系统进行分型。结果:在172个标本中,共检测到DYS392的5个等位基因,基因频率分别为0.0872、0.0756、0.4709、0.2500、0.1163。基因变异度为(GD)0.6929;检测到DYS448的6个等位基因,基因频率分别为0.0116、0.3372、0.3198、0.2674、0.0465、0.0174。基因变异度为0.7141;检测到DYS385的47个单倍型,基因变异度为0.9689。三个位点的基因变异度累计为0.9938。结论:Y-DNA位点:DYS385、DYS392、DYS448是三个具有法医学和群体遗传学应用价值的位点。

Objective：To get the polymorphism data of Y-DNA SIR： DYS385, DYS392. DYS448 in Wenzhou Han population and to apply these date to the human heredity research and forensic medicine. Method：Short tandem repeat （SIR） locus DYS385,DYS392 and DYS448 was analyzed by means of polymerase chain reaction, following polyacrylamide gel electrophoresis system. Result：The gene frequency of the DYS392： 0.0872,0.0756,0.4709, 0.2500 and 0.1163,the gene diversity （GD） was 0.6929.The gene frequency of the YS448： 0.0116,0.3372, 0.3198, 0.2674,0.0465 and 0.0174,the gene diversity waw 0.7141. DYS385 had 47 haplotypes, the gene diversity was 0.9689. The accumulative total gene diversity was 0.9938. Conclusion：The result shows that Y-DNA SIR： DYS385, DYS392 and DYS448 are highly informative and suitable for forensic and population genetics application.