多发性硬化患者外周血单个核细胞IL-2、IFN-γ和TNF-α mRNA表达水平

IL-2,IFN-γ, and TNF-α mRNA Expression in Peripheral Blood Mononuclear Cells in Patients with Multiple Sclerosis

应用逆转录／聚合酶链反应（RT／PCR）和狭缝印迹分子杂交技术，研究17例多发性硬化（MS）患者外周血单个核细胞IL-2、IFN-γ和TNF-α的mRNA表达。结果发现3种细胞因子的mRNA表达均高于健康对照组;治疗后病程处于稳定期的MS患者IFN-γ和TNF-α的mRNA表达恢复到正常水平，但IL-2mRNA仍保持高水平。此结果表明炎性细胞因子（IFN-γ和TNF-α）参与MS发病过程，并可作为MS病情缓解、复发的临床指标。

We determined the cytokine messenger RNA (mRNA) expression patterns of peripheral blood mononuclear cells from 17 patients with multiple sclerosis (MS) and 11 normal controls, by using a quantitative reverse transcription in combination with polymerase reaction (RT/PCR) and Southern blot hybridization method. A significantly higher levels of IL-2,IFN-γ mRNA in patient with MS compared to normal controls and significantly higher level of expressions of IFN-γ, TNF-α mRNA were detected in active MS patients compared to those with stable desease (P＜0. 05). These data further support the central role of some cytokines in the pathophysiology of this autoimmune disease.