一株降解N-酰基高丝氨酸内酯酵母菌菌株的分离鉴定及其降解特性

Isolation and identification of Rhodosporidium toruloides R1 degrading N-acyl homoserine lactone

利用N酰基高丝氨酸内酯(N-acyl-homoserine lactone,简称AHL)为唯一碳源和能源,筛选得到一株能够降解AHL的菌株R1。常规鉴定和18S rDNA序列分析表明,菌株R1属于红冬孢酵母菌(Rhodosporidium toruloides),定名为R.toruloidesR1。结果显示R.toruloidesR1能利用所测试的3种AHL作为唯一碳源和能源生长,具有降解AHL的能力,其对AHL依赖型胡萝卜欧文氏软腐病菌(Erwinia carotovora subsp.carotovora)的致病有一定的抑制作用。

In the present study, a AHL-utilizing strain R1 was isolated and identified as the genus Rhodosporidium toruloides R1 by physi-biochemical approaches and 18S rDNA sequence analysis, and this strain was designated as R. toruloides R1. Results showed that R. toruloides R1 exhibited the ability to utilize and degrade the all N-acyl homoserine lactones tested in this study. Coculture of R. toruloides R1 with Erwinia carotovora subsp. Carotovora effectively inhibit the soft rot disease of patato caused by E. carotovora. To the best of our knowledge, this is the first report on AHL-degradation of yeast cells.