分泌抗 HIVp24 单克隆抗体杂交瘤细胞系的建立及其初步鉴定

Preparation of the monoclonal antibodies against human immunodeficiency virus capsid protein (p24)

以含人免疫缺陷病毒（ＨＩＶ）核心抗原ｐ２４全部氨基酸序列的重组蛋白ｐＧ１免疫小鼠，用免疫脾细胞与ＳＰ２／０骨髓瘤细胞融合，成功地建立了５株分泌抗ＨＩＶｐ２４单克隆抗体（ＭｃＡｂ）的杂交瘤细胞株。经鉴定，这５株ＭｃＡｂ与乙肝核心抗原（ＨＢｃＡｇ），丙肝Ｃ区、ＮＳ３区抗原不发生交叉反应，而与重组ｐ２４抗原产生特异反应。本组单抗的研制成功为建立检测ＨＩＶｐ２４抗原的方法奠定了基础。

Immunizing BALB/c mice with recombinant pG1, the fragment of HIV gag products, we have established five hybridoma cell lines directed against HIV capsid protein p24 with conventional method. Indirect ELISA test demonstrated that they did not have cross reactivity with other viral antigens, such as HBcAg, HCV C, NS3 antigen. They can be applied for the establishment of a test system to detect HIV p24 antigen in serum of patient or blood donor.