热补针法对实验性家兔高脂血症防治作用的研究

Experimental Study on the Prevention and Treatment Effects of Warm-Reinforcing Needling for Hyperlipoidemia in the Rabbit

目的:探讨热补针法对实验性高脂血症家兔血清脂质代谢的影响。方法:将32只青紫兰家兔随机分为4组。每日给模型组、热补组和平补组家兔喂服高胆固醇饲料复制高脂血症模型。治疗组家兔每天同时取“足三里”“三阴交”和“血海”穴分别施以热补针法和平补平泻针法。7周后采血取材,酶法测定血清胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)的含量,以二磷酸腺苷为诱导剂检测血小板聚集率,HE染色观察主动脉内膜斑块变化。结果:热补针法组家兔血清TC含量明显降低,血清HDL含量明显升高,与平补组比较有显著性差异(P<0.05),TG含量和LDL含量明显降低,但与平补组比较没有显著性差异;热补针法抑制血小板聚集和抗动脉粥样斑块形成与平补组比较有显著性差异。结论:热补针法调节血脂和抑制动脉粥样斑块形成的作用优于平补平泻针法,可能与抑制血小板的聚集有关。

Objective： To investigate the effect of warm-reinforcing needling （WRN） on serum lipid metabolism in hyperlipoidemia （HL） rabbits. Methods： A total of 32 rabbits were randomly and evenly divided into control group, WRN group, model group and uniform reinforcing-reducing needling （URRN） group. HL model was established by feeding the rabbits with hypercholesterol and high-fat forage continuously for 7 weeks. For rabbits of WRN and URRN groups, ＂Zusanli＂ （ST 36）, ＂Sanyinjiao＂ （SP 6）, ＂Xuehai＂ （SP 10） were punctured and stimulated manually with WRN and URRN techniques respectively, once daily, continuously for 7 weeks. At the end of experiments, blood samples were collected from the heart, and centrifuged for detecting serum total cholesterol （TC） triglyceride （TG） and high density lipoprotein （HDL） with enzyme method, and platelet aggregation rate （PAR） with an intelligent blood agglutometer. Low density lipoprotein （LDL） =TC- （HDL＋ TG/2.2）. Results： Compared with normal control group, serum TC, TG and LDL contents in model group, WRN and URRN groups increased significantly （P〈0.01）, and HDL content of model group decreased considerably （P〈0.01）; while in comparison with model group, serum TC, LDL and TG contents of WRN and URRN groups decreased considerably （P〈0.01）, and serum HDL levels of WRN and URRN groups increased significantly （ P〈0.01 ）. Serum TC content of WRN group was markedly lower than that of URRN group （P〈0.05）, and HDL of WRN group was remarkably higher than that of URRN group （ P〈0.01 ）. In comparison with normal control group, 1 min, 5 min and maximum PARs in model, WRN and URRN groups increased significantly （P〈0.01 ）, while in comparison with model group, 1 min, 5 min and maximum PARs of WRN and URRN groups decreased considerably （P〈0.01）. In addition, 1 min and maximum PARs in WRN group were markedly lower than those in URRN group （P〈0.05）. Light microscopic observation showed that the apoptotic foam cells in the lipid plaque of aorta wall in model group appeared and were obviously more than those of WRN and URRN groups. Conclusion： Acupuncture can effectively lower serum TO, LDL and TG levels, raise serum HDL content, and suppress platelet aggregation, and the therapeutic effect of WRN is significantly superior to that of URRN,which may contribute to the action of acupuncture in regulating blood lipid and inhibiting the formation of atheromatous plaque.