电针对动情周期不同阶段大鼠生殖内分泌轴的调节作用

Regulative Effect of Electroacupuncture on Hypothalamus-Hypophysis-Gonad Axis in Different Stages of Estrous Cycles in Rats

目的:探讨电针调节生殖内分泌相关激素的特异反应穴位、最佳治疗时机及作用途径。方法:①选用成熟雌性有正常动情周期的SD大鼠,根据阴道涂片确定动情周期所处阶段。设假电针组、关元组、内关组,每个组当中大鼠分别处于动情间期、动情前期、动情期和动情后期。分别在电针治疗前、电针30min后即刻、出针30min从髂外静脉采血,用放射免疫法检测血清促性腺激素释放激素(GnRH)和黄体生成素(LH)浓度。②设损毁组(损毁正中隆起)和假损毁组,观察电针“关元”穴对两组大鼠血中GnRH、LH和雌二醇(E2)的调节规律。结果:①从假电针组可以看出:动情周期不同阶段GnRH和LH水平是不同的,GnRH水平在动情期最高,LH水平在动情前期最高;GnRH和LH的含量变化是同步的;②针刺可以使血中GnRH和LH水平增加,以关元穴组效果明显,在动情间期和动情后期表现更明显;③针刺对GnRH和LH的调节作用表现出时间性,30min比60min明显;④损毁正中隆起后电针对血中GnRH水平仍有一定的调节作用,但对LH和E2水平的调节作用基本消失。结论:相同穴位在动情周期不同阶段对GnRH和LH的调节程度不同,以动情间期和动情后期表现更明显;不同穴位在动情周期相同阶段对GnRH和LH的调节作用不同。针刺对与生殖内分泌相关激素的调节作用主要是通过下丘脑-垂体-性腺轴实现的。

Objective： To investigate the specific acupoint, optimal curative opportunity and the involved pathway of electroacupuncture （EA） for regulating gono-endocrine hormones. Methods： ①Adult female rats were randomly divided into sham-EA group （n = 32）, ＂Guanyuan＂ （CV 4） group （n = 36） and ＂Neiguan＂ （PC 6） group （n = 36） which were further divided into diestrum, preoestrus （P-EST）, estruation and metaoestrus subgroups according to tetra-estrous cycles determined by vaginal smear test. EA （20/60 Hz, 2-3 mA） was applied to CV4 and PC6 separately for 30 min from about 9：30 to 10：00 Am. Blood samples were collected from the common lilac vein before, 30 min and 60 min after EA for detecting serum genadetropin-releasing hormone （GnRH） and luteotropic hormone （LH） contents with radioimmunoassay. ②Other female rats were randomly divided into median eminence （ME） lesions group and control group, which were used to observe the influence of hypothalamic ME lesion on the effect of EA of CV4 in regulating GnRH, LH and estradiol （E2） levels. Results： ① Before the treatment, serum GnRH and LH levels were slightly different in different stages of the estrous cycle, GnRH level was highest in the estruation and LH content highest in the precestrus. ② After EA, both serum GnRH and LH levels increased to a certain degree at most stages. Compared with sham-EA group, GnRH levels in the diestrum and metacestrus and LH in all the 4 stages of the estrous cycle in CV4 group increased significantly （P〈0. 05, 0.01 ）. Self-comparison showed that after EA of CV4, serum GnRH level in diestrum and metacestrum, and LH contents in diestrum and metaoestrus increased markedly （P〈0.05,0.01 ） ; while after EA of PC6, serum GnRH and LH levels in diestrum and metacestrum increased slightly, suggesting that the effect of EA of CV4 was superior to that of EA of PC6 in promoting secretion of GnRH and LH.③Thirty minutes after EA, self-comparison between pre-and post-EA in each group and comparison among 3 groups showed no significant differences in both serum GnRH and LH levels （P〉0.05）. ④Electrolytic lesion weakened the effects of EA in promoting the secretion of GnRH, LH and E2, though the influence of this lesion on GnRH secretion was milder. Conclusion ： EA can enhance the secretion of GnRH and LH in the diestrum and metaoestrus of the estrous cycle, which is realized by the hypothalamus-pituitary-gonad axis. The effect of EA of CV4 is better than that of PC6.