针刺“足三里”对脾虚证大鼠血清中胃泌素、皮质醇含量的影响

Effect of Acupuncture of “Zusanli”(ST 36)on Serum Gastrin and Cortisol Contents in Rats with Spleen Deficiency Syndrome

目的:探讨针刺“足三里”治疗大鼠脾虚证的作用机理。方法:选用成年雄性Wistar大鼠26只,随机分为4组。其中正常对照组6只,脾虚造模组8只,自然恢复组6只,针刺治疗组6只。除正常对照组外,其余3组均按“彭式”方法建立脾虚证模型。针刺“足三里”治疗11d后,断头取血,用放射免疫方法测定血清中胃泌素(Gas)、皮质醇(Cor)的含量。结果:针刺“足三里”穴可使脾虚大鼠血清中Gas、Cor的水平升高,明显高于模型对照组(P<0.05);自然恢复组与模型对照组比较血清中Gas、Cor含量没有明显变化(P>0.05);自然恢复组血清中Gas、Cor含量明显低于正常对照组和针刺治疗组(P<0.05)。结论:针刺“足三里”治疗脾虚证与其对Gas、Cor等激素水平的调节作用有关。

Objective： To study the underlying mechanism of acupuncture of ＂Zusanli＂ （ST 36） in the treatment of spleen deficiency syndrome. Methods： Twenty-six adult Wistar rats were randomly divided into normal control group （n = 6）, model group （n = 8）, saline group （n = 6） and acupuncture group （n = 6）. Spleen deficiency syndrome model was duplicated by intragastric perfusion of 50% alcohol （on the 1^st day, 1 mL/100 g） and white vinegar for 9 days （1 mL/100 g）. Before decapitation, the animals in all the 4 groups were forced to swim in a water pool under temperature of 18 ℃ till exhaustion （sinking to the bottom of the water pool）. Blood samples were collected after decapitation of rats for detecting contents of serum gastrin and cortisol with radioimmunoassay. Unilateral ＂Zusanli＂ （ST 36） was punctured and stimulated manually then the needle was retained for 20 min. The treatment was given once daily and continuously for 10 days. Results： Compared with normal control group, serum gastrin and cortisol contents of model and saline groups decreased significantly （P〈0.05）, while compared with model group and saline group, both gastrin and cortisol levels increased remarkably in acupuncture group （P〈0.05）. No significant differences were found between saline group and model group, and between normal control group and acupuncture group in serum gastrin and cortisol levels （P〉0.05）. Conclusion： Acupuncture of ＂Zusanli＂ （ST 36） can suppress alcohol and vinegar induced decrease of serum gastrin and cortisol, which may contribute to its effect in the treatment of spleen deficiency syndrome in the rat.