复合转化生长因子-β的组织工程化周围神经修复坐骨神经缺损

Tissue Engineering Peripheral Nerve with TGF-β Repair Sciatic Nerve Defect

探讨了转化生长因子-β(Transforming growth factor-β,TGF-β)在周围神经缺损修复中的作用。将50 ng·ml-1 TGF-β加入体外培养的雪旺细胞(Sehwann cells,SC)中,MTT和流式细胞仪观测到TGF-β能够明显促进SC增殖;ELISA方法检测到TGF-β组上清神经生长因子(NGF)含量高于对照组(P<0．05);将牛去细胞基质(Bovine acellular matrix,BAM)、SC、血清和培养基按一定的比例混合,注入聚乳酸羟基乙酸共聚物(PLGA)神经导管去修复15 mm坐骨神经缺损。30只SD大鼠分为3组,实验组:PLGA导管+SC+TGF-β;空白组PLGA导管+SC;和自体神经移植组。16周后通过电生理、透射电镜等检测方法显示各组坐骨神经均得到再生,修复效果实验组与自体神经移植组无显著性差异,均优于空白组。TGF-β一方面可以明显促进SC的增殖,另一方面可以增强SC分泌NGF的功能。因此周围神经修复过程中使用外源性的TGF-β对修复周围神经缺损有较好的疗效。

The purpose of this investigation was to evaluate the effect of transforming growth factor-β（TGF-β） on the efficacy of Schwann cell （SC） and on the repair of peripheral nerve defect. 50 ng · ml^-1 TGF-β was shown to promote the proliferation of SC by MTT and flow cytometry （FCM） assay, and NGF synthesis in SC culture media was noted to be of significantly higher concentration by ELISA method（P〈0.05）. SCs mixed with bovine acellular matrix（BAM）,fetal bovine serum and media based on definite ratio were injected into polylactideco-glycolide acid （PLGA）guide. 30 SD rats, each had a man-made sciatic nerve defect 15 mm long, were randomly divided into 3 groups ： experiment group （PLGA conduit ＋ SC＋ TGF-β）, control group （PLGA conduit＋ SC）, and autograft group. After 16 weeks, it was demonstrated that the effect of the test group was not significantly different from that of the autograft group,but it was better than that of the control group by means of electrophysiological test and sciatic nerve function index （SFI）. TGF-β can promote not only the proliferation, but also the NGF synthesis of SC obviously. The use of exogenous TGF-β in the repair of peripheral nerve defect may produce better curative effect.