常见革兰阳性细菌鉴定与耐药检测基因芯片的临床应用

The Clinical Application of DNA Chip for Common Gram-positive Bacteria Identification and Drug Resistance Detection

目的利用基因芯片技术进行常见革兰阳性细菌的细菌鉴定和耐药性检测。方法用生理生化鉴定和药敏纸片法确定445份革兰阳性细菌菌株的细菌种类和耐药性,盲法进行革兰阳性细菌鉴定与耐药检测基因芯片的检测,用SPSS 10.0 for Windows软件统计分析。结果与临床常规方法相比,耐药检测基因芯片鉴定指标的灵敏度均>96%,特异度均>98%;耐药检测指标的灵敏度均>94%,特异度均>90%。χ2检验结果P值均>0.05,基因芯片检测结果与临床常用方法检测结果差异无统计学意义。Kappa值均>0.75,2种方法一致性较好。结论基因芯片技术对细菌鉴定和耐药检测有一定的临床应用价值。

Objective To study the clinical application of DNA chip for common Gram-positive bacteria identification and drug resistance detection. Methods 445 Gram-positive strains were identified with physiological and biochemical methods, and were detected of drug resistance with diffusion disk method for susceptibility test. Then the strains were blindly detected with using DNA chip for identification and drug resistance. The data were analyzed using SPSS 10.0 for Windows software. Results The sensitivity of all identification indexes of DNA chip were higher than 96%, and all of their specificity were higher than 98%. The sensitivity of all drug resistance indexes of DNA chip were higher than 94%, and all of their specificity were higher than 90%. The chi square test values of all indexes were higher than 0.05, showing that there was no significant difference between DNA chip and the clinical routine methods. The Kappa values of all indexes were higher than 0.75, indicating that the coincidence between the two kinds of methods was good. Conclusion There is clinical potential of gene chip technology for bacteria identification and drug resistance detection.