万古霉素耐药肠球菌基因检测及同源性分析

Resistant Genes Detection and Homologous Analysis ofVancomycin Resistant Enterococcus

目的检测肠球菌万古霉素耐药基因,分析万古霉素耐药肠球菌耐药表型及基因同源性。方法用琼脂扩散法检测肠球菌对万古霉素及其他抗菌药物的敏感性,用PCR检测万古霉素耐药基因,用随机扩增DNA多态性分析(Random amplified polymorphic DNA method,RAPD)对万古霉素耐药肠球菌分型。结果检测到4株万古霉素耐药屎肠球菌,6株万古霉素耐药鹑鸡肠球菌,均携带vanA基因,经RAPD分析,可将10株肠球菌分为7个基因型。结论肠球菌万古霉素高水平耐药主要由vanA介导,RAPD技术是进行肠球菌同源性分析的有效方法之一。

Objective To detect vancomycin-resistant genes of enterococcus, and to analyses antibiotic resistant phenotype and genotype. Methods Susceptibility of vancomycin and other antibiotics were performed with agar diffusion methods. Vancomycin resistant genes and homologous analysis were detected with PCR and RAPD methods, respectively. Results 6 strains of Enterococcus gallinarum and 4 of Enterococcus faecium were identified in the study, all of which carried vanA gene. According to RAPD results, 10 strains were divided into 7 genetypes. Conclusion Vancomycin resistance in enterococcus is mainly mediated by vanA. RAPD is the effective methods to analyze the homogeneity of vancomycin resistant enterococcus.