高压氧促进成年大鼠神经干细胞的增殖

Hyperbaric oxygen stimulates NSCs proliferation in adult rats

目的:研究高压氧对成年大鼠神经干细胞增殖的影响。方法:采用BrdU免疫组化方法,观察高压氧对成年大鼠神经干细胞增殖的影响,同时通过脑内注射内皮素制备大脑中动脉闭塞(MCAO)模型,对这两种诱导神经干细胞增殖的处理加以比较。结果:高压氧暴露10d,侧脑室前角和外侧壁的BrdU^+细胞数量显著增加,BrdU^+细胞核计数显著多于正常组,阳性细胞核反应性也显著增强,多为深染的棕黑色细胞核,其效应与MCAO第8天对BrdU^+细胞的效应类似;高压氧暴露10d也引起了海马齿状回颗粒细胞层BrdU^+细胞数量显著增加。结论:反复高压氧暴露可能具有调节脑内神经干细胞增殖与分化的作用。

Objective： To study the effects of hyperbaric oxygen （HBO） on neural stem cells （NSCs） proliferation in adult rats. Methods： BrdU immunohistochemistry was used to study the influence of HBO on the proliferation of NSCs in adult rats. Meanwhile, a middle cerebral artery occlusion （MCAO） model induced by Endothelin-1 injection was used to compare the effects on inducing NSCs proliferation. Results： After a 10-day exposure to HBO, BrdU positive cells located at subventricular zone were significantly increased （ P 〈 0. 001 ） and most of them showed dark-stained nuclei, which was comparable with the influence caused by MCAO. HBO also induced an increase of BrdU positive cells in the dentate gyrus of hippocampus （ P 〈 0. 05 ）. Conclusion： Repeated hyperbaric oxygen exposure probably regulates the proliferation and differentiation of the neural stem cells.