苯那普利和缬沙坦联合应用对大鼠阿霉素肾病的影响

Combination therapy with Benazepril and Valsartan in rats with adriamycin-induced nephropathy

目的:探讨苯那普利和缬沙坦对大鼠阿霉素肾病的干预作用。方法:实验Wistar大鼠实行右肾切除手术,以阿霉素尾静脉注射造模,分别用苯那普利、缬沙坦及两者联合灌胃。用药后2～8周检测血、尿标本;透射电镜下观察肾形态学变化;用TUNEL法检测肾组织细胞凋亡。结果:与肾病组相比,3个干预组中的肌酐、尿素氮、尿蛋白、血压均降低,且差异显著;电镜下肾小球超微结构改善;凋亡细胞减少,差异显著。联合应用组与单用组差异显著。结论:苯那普利、缬沙坦及两者联合应用均可延缓阿霉素肾病肾小球硬化的进展,其机制可能与减少细胞的凋亡及肾超微结构的改善有关,两药联合应用效果更显著。

Objective： To investigate the protective effects of Benazepril （ACEI） and Valsartan （ARB）, and combination of them on adriamycin-induced nephropathy in rats. Methods： Experimental model in uninephrectomized rats was made by adriamycin injection. Rats were treated with Benazepril or Valsartan or combination of them. Two to eight weeks after treatment, 24 h urinary protein, serum creatinine （Scr）, blood urea nitrogen （BUN） and blood pressure were detected. The morphologic changes of the kidneys were studied by electronic microscopy. Apoptotic cells in the kidneys were analyzed. Results： The content of Scr, BUN and 24 h urinary protein, blood pressure, and changes of glomerular ultrastructure in three treated groups were much lower than those in the nephropathy group. The apoptotic cells in three treated groups were significantly decreased. There were obvious additional protective effects in the group treated by the combination of ACEI and ARB. Conclusion： Combination of ACEI and ARB has protective effects on adriamycin-induced nephropathy, probably by reducing the apoptotic cells and improving the glomerular ultrastructure.