大鼠烫伤后小肠上皮内T细胞的变化

Changes of T lymphocytes in small intestine epithelium in scalded rats

目的:探讨大鼠烫伤后小肠上皮内TCRαβT细胞和TCRγδT细胞在各肠段问的变化。方法:将大鼠随机分成正常对照组,烫伤后1、3和7d组。除正常对照组,其余的制作全身体表面积(TBSA)30%Ⅲ°烫伤模型。采用免疫组化和体视学测量方法,分别测出其T细胞数密度、平均体积和表面积体积比,并比较它们在肠段间的差异。结果:烫伤后(除回肠TCRγδT细胞)肠黏膜T细胞数密度均下降,第3天最低。无论正常或烫伤后,TcRγδT细胞平均体积均比TCRαβT细胞增大。结论:烫伤后(除回肠TCRγδT细胞)肠黏膜T细胞的数量均减少,第3天最少,但其平均体积和形状规则程度与烫伤前相比没有显著变化。

Objective： To explore the changes of the distributional law of TCRαβT cells and TCRγδT cells in the epithelium of different small intestines in scalded rats. Methods： Forty male Wistar rats were randomly divided into four groups （n=10）： control group, postburn 1 d （24 h） group, postburn 3 d （72 h） group and postburn 7 d group. Except the control group, the other three experimental groups were inflicted with 30% total body surface area （TBSA） Ⅲ° scald. Using ABC immunohistochemical technique and stereological methods, the number density （Nv）, mean volume （ V ） and Rsv were measured. Results： Nv of T cells in the epithelium of the small intestine decreased with time after being scalded, except Nv of TCRγδT cells in the epithelium of the ileum. Whether the rats were scalded or not, Ⅴ of TCRγδT cells were larger than those of TCRαβT cells. Conclusion： The amount of T cells in the epithelium of the small intestine decreased with time after being scalded, except TCRγδT cells in the epithelium of the ileum. The volume and shape of T cells in the epithelium after being scalded were the same as those of the control group.