孕酮对沙土鼠脑缺血再灌海马小白蛋白免疫反应的影响

Effects of progesterone treatment on parvalbumin immunoreactivity of the gerbil hippocampus after cerebral ischemic reperfusion

目的:观察孕酮对沙土鼠脑缺血再灌海马小白蛋白免疫反应的影响。方法:以双侧颈总动脉夹闭法制作脑缺血再灌注模型,免疫细胞化学方法显示海马小白蛋白免疫反应的变化。结果:脑缺血再灌后1～7 d,海马小白蛋白阳性神经元增多;第7天背侧海马CA1区锥体细胞层呈现小白蛋白阳性致密条带。脑缺血再灌孕酮处理后小白蛋白阳性神经元进一步增加,可见许多串珠状小白蛋白阳性突起,未见海马CA1区锥体细胞层小白蛋白阳性致密条带结构。结论:孕酮可能通过对小白蛋白的表达调控发挥对脑缺血再灌损伤的部分神经保护作用。

Objective： To investigate the effects of progesterone treatment on parvalbumin （PV） immunoreactivity of the gerbil hippocampus after cerebral ischemic reperfusion. Methods： Cerebral ischemic reperfusion was produced by bilateral occlusion of common carotid arteries and immunocytochemistry were used to reveal PV immunoreactivity. Results： Cerebral ischemic reperfusion increased the number of PV immunoreactive （IR） neurons of hippocampus at 1-7 day, and PV-IR dense strip was observed in CA1 pyramidal stratum at 7 th day. Progesterone treatment after ischemic insult further increased the number of PV-IR neurons of hippocampus. A lot of long, beaded PV-IR processes were found in hippocampus and no PV-IR dense strip was observed in CA1 pyramidal stratum after progesterone treatment. Conclusion： The protective effect of progesterone on cerebral ischemic reperfusion may partially be induced by upregulation of PV expression in PV-IR neurons.