细胞因子对鸡胚胎原始生殖细胞(EPGCs)增殖的影响

Effect of Exogenous Cell Factors on Chicken EPGCs Proliferation

采用MTT法分别检测mLIF、bFGF、hSCF、hIL-11四种细胞因子协同作用对体外培养的第19、28期鸡EPGCs生长的影响。结果表明与对照组相比较,19期的EPGCs体外培养72h后,mLIF、hSCF、bFGF、hIL-11对鸡EPGCs的增殖影响显著（P〈0.05）。mLIF的最佳作用剂量是10-20ng/ml,hSCF的最佳作用剂量是15-20ng/ml,bFGF的最佳作用剂量是10-20ng/ml。hSCF、bFGF的联合使用优于单因子作用的结果（P〈0.05）。单独使用hIL-11时,细胞的增殖情况比其他三因子单独使用的效果较差,但OD均值有随剂量增高而上升的趋势。在与其他因子联合使用的情况下,hIL-11的最佳作用剂量为0.10-0.20ng/ml。

The effect of exogenous cell factors as mLIF, hSCF, bFGF and hIL-11 on the stage of the 19th and 28th EPGCs proliferation and differentiation were elucidated by means of MTT test when combined added in the culture medium. The regulated mechanism of those cell factors was discussed as well. When the cell factors as mLIF, hSCF, bFGF and hIL-11 combined in the medium, it showed evident effect on proliferation of stage 19th EPC, Cs compared to the control group when cultured for 72h （ p 〈 0.05 ）. The proliferation of EPGCs was obviously inhibited when the concentration of mLIF and hSCF beyond 20ng/ml, the suitable concentration of mLIF was 10 -20ng/ml,and hSCF was 15 -20ng/ml. The combined of hSCF and bFGF was more efficient than single cell factor. The suitable concentration of bFGF was 10 - 20ng/ml. Similar to hIL-11, when combined with the other cell factors, the number of viable cells increased distinctly （ P 〈 0.05 ）, and the suitable concentration of hIL-11 was 0.10 - 0.20ng/ml. The results would promote and facilitate development of chicken EPGCs line and also highlight a general role in stem cells proliferation mechanism.