摘要
首次分离得到黑盖木层孔菌发酵液粗多糖(PNM),糖含量达到49.0%,对小鼠离体淋巴细胞有极显著的增殖作用。PNM经冻融分级、Sevag法脱蛋白、SepharoseCL-6B柱层析得单一级份(PNMⅠ),分子量约29kDa。气相色谱分析PNMⅠ为一杂多糖,主要由甘露糖、半乳糖、葡萄糖组成。初步考察,PNMⅠ对小鼠离体淋巴细胞也有极显著的增殖作用,可以抑制小鼠体内S-180肉瘤的生长。
The water-soluble polysaccharide PNM isolated from the submerged culture of PheUinus nigiricans by hot water extraction and ethanol precipitation, was purified by freeze thawing, methods of Sevag, and then fractionated by Sepharose CL-6B gel filtration chromatography, giving a polysaccharide fraction named as PNMI. HPLC analysis showed that PNMIwas a homogeneous polysaccharide, with a weight-average molecular weight of 29 kDa. GC analysis indicated that PNMIis a heteropolysaccharide composed of mannose, galactose and glucose with a ratio of 0. 35：0. 44： 1.00. Pharmaceutical experiments showed both PNM and PNMI have a significant effect on the immunomodulatory activities of murine splenic lymphocytes in duo. Also PNMI could inhibit the growth of sarcoma 180 solid tumor in vivo.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2007年第4期50-53,共4页
China Biotechnology
基金
吉林省科学技术厅基金资助项目(20060559)
关键词
黑盖木层孔菌
多糖
分离纯化
淋巴细胞转化
Phellinus nigiricans Polysaccharides Isolation and purification Murine splenic lymphocytes
