摘要
目的:探讨AD大鼠海马4-羟基壬烯醛(HNE)含量的改变及维生素E对其的影响。方法:将SD大鼠36只随机均分为假手术组、AD模型组、维生素E治疗组三组。以β-淀粉样蛋白(Aβ25-35)左侧杏仁核注射制备AD大鼠模型,用Y迷宫检测并比较三组大鼠学习记忆功能。术后4周(维生素E治疗组服用维生素E后14天),应用免疫组化方法测定并比较三组大鼠海马中HNE的含量。结果:(1)术后4周,维生素E治疗组学习记忆能力较假手术组(P<0.05),但较AD模型组有改善(P<0.05)。(2)术后4周,维生素E治疗组海马中HNE含量多于假手术组(P<0.01),但少于AD模型组(P<0.01)。结论:AD模型大鼠海马中HNE含量增多;维生素E可以通过清除自然基的方式,减少脂质代谢产物的聚集,抑制氧化应激,改善AD症状。
Objective： To study influences of Vitamin E on contents of 4- hydroxy- nonnenal （HNE） in the hippoeampus of AD rots.Methods：Thirtysix SD rats were divided into three groups, ineluding sham operation,AD model and Vii E treatment group. The AD rat models were established by injecting amyloid - beta protein （ Aβ25-35） into left amygdalae. These rats＇ learning and memory ability were observed and compared by Y- maze detection. In postoperative four weeks （namely,on 14th day of Vit E treatment）,the HNE contents of hippocampus tissues in the three groups were evaluated by immunohistochemieal method and compared to each other. Results： Our findings indicated that,after 4 weeks of operation,the rats＇ learning and memory abilities in Vit E treatment group were decreased than those in sham operation group （ P 〈 0.05）, but improved than AD model group（ P 〈 0.05） ; in contrast, HNE contents of hippocampus tissues in Vit E treatment group exceeded sham operation group （ P 〈 0.01 ）, but less than AD model group （P 〈 0.01）. Conclusions：Vitamin E could decrease accumulation of lipid metabolites, inhibit oxidative stress and improve symptoms of AD by scavenging free radicals.
出处
《解剖与临床》
2007年第2期98-100,共3页
Anatomy and Clinics
