粘着斑激酶在增生性瘢痕成纤维细胞中的表达和意义

Expression and significance of focal adhesion kinase in hypertrophic scar fibroblasts

目的探讨粘着斑激酶(FAK)在增生性瘢痕成纤维细胞(HSFB)中的表达和意义。方法运用细胞免疫组化技术,测定HSFB和正常皮肤成纤维细胞(NSFB)中的FAK、整合素α1、转化生长因子受体1(TGF-βR1)及α肌动蛋白(α-SMA)的表达;同时将HSFB分别用抗FAK、整合素α1、TGF-βR1及α-SMA抗体进行阻断培养,并测定培养前后整合素α1、TGF-βR1、α-SMA及FAK的表达。结果与NSFB相比,HSFB中的FAK、整合素α1、TGF-βR1、α-SMA的表达增高,差异具有显著意义(P<0.05)。分别用抗整合素α1、TGF-βR1、α-SMA抗体进行阻断培养后,HSFB中的FAK表达下降(P<0.01);阻断FAK表达也可分别导致了整合素α1、TGF-βR1、α-SMA的表达降低(P<0.01)。结论FAK对整合素α1、TGF-βR1及α-SMA的蛋白合成具有重要的调控作用,与增生性瘢痕的发生、发展关系密切。减少FAK在成纤维细胞中的过度表达,可能是抑制瘢痕增生、软化瘢痕的新途径。

Objective To study the expression and mechanism of focal adhesion kinase （FAK） in hypertrophic scar fibroblasts （HSFB）. Methods Immunocytoehernistry was applied to detect the expression of FAK, integrionα1, TGF - βR1, and α - SMA in hypertrophic scar and in human fibroblasts from normal skin. The expression of FAK, integrinα1, TGF - βR1, and α - SMA was evaluaed with and without antibodies to FAK or integrinα1 or TGF- βR1 or α - SMA. Results The expression of FAK, integrinα1, TGF - βR1 and α- SMA in hypertrophic scar fibroblasts was significantly higher than that in the nomal skin fibroblasts （P〈0.05）. The expression of FAK in hypertrophic scar fibroblasts was reduced after blocking integrinα1, TGF- βR1, α- SMA （P〈0.01）, separately, and the expression of integrinα1, TGF - βR1, α - SMA was reduced after blocking FAK （P〈0.01）. Conclusion FAK is the key regulation point of protein synthesis of integrinα1, TGF- βR1 and α - SMA. It may be closely related to the proliferation and contracture of hypertrophic sear. Suppressing the over- expression of FAK in hypertrophic scar fibroblasts may be a new way to control the proliferation and contracture of hypertrophic scar.