转化生长因子β的提取、纯化及其促进骨生长作用的实验观察

Extraction and Purification of TGF β and Experimental Observation of Its Bone Growth Stimulating Effect

转化生长因子β（ＴＧＦ－β）具有广泛的生物学作用，其在血小板中的含量相对较高。本实验从新鲜牛血小板中利用酸／醇抽提，离子交换色谱和凝胶色谱纯化获得分子量为２５ｋＤ的ＴＧＦ－β，并观察了其诱导肉芽组织形成和促进骨生长的作用。结果发现，皮下注射ＴＧＦ－β可以诱导局限性肉芽组织形成。另外，ＴＧＦ－β还可刺激骨膜内间充质细胞的增殖、分化，软骨形成和软骨化骨。

TGF β exhibits a remarkable diversity of biological activity. Platelets are a major storage site for TGF β. The aims of this study were to isolate TGF β from bovine platelets and demonstrate its biologic activity. TGF β was isolated from bovine platelets by acid/ethanol extraction, cation exchange and gel filtration chromatography. As determined by SDS PAGE, the protein was mainly 25 kD. TGF β was injected subcutaneously in mice. The blocks of multipore ceramic bovine bone(CBB) were prepared and selected as the carrier for TGF β. The complexes of TGF β and CBB were implanted into the thigh muscle of mice. Results showed that TGF β caused formation of granulation tissue at the site of injection. TGF β could also stimulate proliferation and differentiation of mesenchymal cells within the periosteum, chondrogenesis and endochondral osteogenesis. These data demonstrate that TGF β plays an important role in soft tissue repair, bone fracture healing and bone defect repair.