加味四逆散对刀豆蛋白A所致免疫性肝损伤的保护作用

Protective effect of Jiaweisinisan decoction on liver injury induced by cocanavalin A in mice.

目的观察加味四逆散对刀豆蛋白A(Con A)所致肝损伤的保护作用。方法选择60只健康NIH小鼠,随机分为正常对照组、模型对照组、联苯双酯组和加味四逆散大、中、小剂量组。除正常对照组外,其他各组于实验首日静脉注射ConA(20mg/kg),联苯双酯组按150mg/kg灌胃,加味四逆散大、中、小剂量组分别按40g/kg、20g/kg和10g/kg灌胃,每天1次,连续3d。末次给药后4h再次尾静脉注射ConA(20mg/kg),8h后检测血清ALT活性,IFN-γ和TNF-α含量。结果加味四逆散大、中剂量组比小剂量组小鼠ALT活性明显降低,加味四逆散治疗组血清IFN-γ、TNF-α含量明显低于模型组,差异有显著性(P<0.05,P<0.01)。结论大、中剂量加味四逆散对刀豆蛋白A所致小鼠肝损伤具有较好的保护作用,其机制与其降低IFN-γ,TNF-α等炎症介质含量密切相关。

Objective To observe the protective effect of Jiaweisinisan decoction on hepatic lesions in mice induced by cecanavalin A （Con A）. Methods Sixty NItt mice were randomly divided into 6 groups： normal control group （group A）, model control group （group B）, Bifendate control group （group C）, JWSNS large dose group （group D）, middle dose group （group E） and small dose group （group F） . Excluded mice in group A,all mice in other groups were injected with Con A 20 mg/kg via the tail vein at the first day of experiment. Group C were administered with Bifendate by gastric perfusion at the dose of 150mg/kg. Group D, group E and group F were administered with JWSNS bifendate by gastric peffusion at the dose of 40g/kg,20g/kg, 10g/kg respectively. All the drugs were given one time a day for 3 successive days . ConA 20mg/kg was injected into the mice four hours after last administration and the activity of serum aminotransferase （ALT）, the contents of interferon - gamma （IFN - γ） and tumor necrosis factor alpha （TNF-α） were determined 8 hours after injection of ConA. Results The activity of ATL in mice of large and middle JWSNS dose groups dropped, the contents of senma IFN -γ and TNF - α in JWSNS groups were significantly lower than that of model control group （P 〈 0.05, P 〈 0.01）. Conclusion JWSNS in large and middle doses is effective in protecting hepatic lesions in mice induced by ConA and this mechanism may be closely associated with its action in bring down the inflammatory mediator of IFN - γ and TNF- α.