产妇血浆和新生儿尿液HCMV-DNA检测分析

Analysis of human cytomegalovirus DNA concentration in materal plasma and peripheral blood mononuclear cells and their neonatal urine.

目的了解产妇血浆、外周血单个核细胞(PBMC)和新生儿尿液中人巨细胞病毒核酸(HCMV-DNA)含量,并探讨其相关性。方法应用荧光定量聚合酶链反应(FQ-PCR)检测42例分娩了新生儿黄疸、早产儿和低出生体重儿的产妇血浆、PBMC及其新生儿尿液中HCMV-DNA的含量(观察组),同时检测36例正常新生儿尿液及其产妇血浆和PBMC中HCMV-DNA的含量(对照组)。结果观察组产妇血浆、PBMC和新生儿尿液中HCMV-DNA的阳性率分别为45.2%(19/42)、64.3(27/42)和61.9%(26/42),与对照组的阳性率5.6%(2/36)、11.1%(4/36)和8.3%(3/36)相比有统计学意义(χ2=15.5,P<0.01、χ2=22.9,P<0.01和χ2=25.2,P<0.01);观察组HCMV-DNA的平均含量(105.1±2.1)copies/ml(不含阴性)与对照组(104.4±1.4)copies/ml(不含阴性)相比(取10的对数)有统计学意义(t=1.70,P<0.05)。观察组26例产妇的PBMC与新生儿尿液中HCMV-DNA同时阳性时,两者HCMV-DNA含量呈正相关(r=0.758,P<0.01)。结论HCMV的感染与新生儿黄疸、早产和低体重患儿密切相关;新生儿尿液作为HCMV感染的检测标本,具有无创伤、易留取和真实反映体内感染情况的优点。

Objective To survey the concentration of human cytomegalovirus （HCMV） DNA in maternal plasma and peripheral blood mononuclear cells（PBMC） and in neonatal urine, and explore their correlation. Methods Fluorescent quantitative polymerase chain reaction （ FQ - PCR） was applied to determine the concentration of HCMV - DNA both in the plasma and PBMC of 42 mothers that delivered jaundice, premature and low weight neonates and in the urine of their neonates （ observation group）. Meanwhile, the concentration of HCMV- DNA in 36 normal maternal plasma and PBMC and their normal neonatal urine were determined （control group）. Results The positive rate of plasma and PBMC of mothers and urine of neonates in the observation group were 45.2% ,64.3 and 61.9% respectively significantly higher than that of the control group （χ^2= 15.5, P 〈0.01 ;χ^2= 22.9, P 〈0. 01 and χ^2= 25.2, P 〈 0.01 ）. In addition, the medial concentration of HCMV - DNA in the observation group was 105.1 ± 2. leopies/ml higher than that of the control group （ t = 1.70, P 〈 0.05）. There 26 cases were positive both for HCMV- DNA in lying -in women and PBMC in the neonates in the observation group. The correlations HCMV- DNA of lyingin women and their neonates were positive correlated （r = 0.758, P 〈 0.01）. Conclusion HCMV infection is closely correlated with jaundice, premature and low weight of the neonates. The determination of neonatal urine samples is convenient and without trauma and truly reveal the actual status of HCMV - DNA infection.