亚低温添加拉莫三嗪对缺血缺氧性脑损害的神经保护作用

Neuroprotective effects of mild hypothermia plus lamotrigine on hypoxicischemic brain damage in neonatal rats.

目的研究亚低温添加拉莫三嗪(LTG)对缺氧缺血性脑损伤(HIBD)新生大鼠的神经保护作用。方法采用左侧颈总动脉离断并置入密闭缺氧箱,制备HIBD动物模型。将56只SD大鼠随机分为4组(每组14只):常温组;亚低温组,缺血缺氧后立即诱导亚低温并持续12h;拉莫三嗪组,脑缺血后立即按20mg/kg剂量腹腔注射拉莫三嗪;亚低温添加拉莫三嗪组,脑缺血后按20mg/kg剂量腹腔注射拉莫三嗪,诱导亚低温并持续12h。用酶标法、免疫组化法,分别检测HIBD 24h后血清神经元特异性烯醇化酶(NSE)浓度、皮层NSE阳性细胞数。结果与亚低温组比较,亚低温添加拉莫三嗪组血清NSE的浓度显著降低,皮层NSE阳性细胞数显著增多。结论亚低温添加拉莫三嗪对缺血缺氧性脑损害具有协同保护作用。

Objective To investigate the neuroprotective effects of mild hypothermia plus lamotrigine （LTG） on hypoxic- ischemic brain damage （HIBD）. Methods HIBD model was prepared by ligation of the left common carotid arteries for 2 hours in postnatal 7 days SD rats, and then the rats were exposed to 8% of oxygen and 92% of nitrogen for 2 hours. The rats were divided into 4 groups each consisted of 14 including the nomothermia group, the mild hypothermia group in which mild hypothermia were induced after HIBD and lasted for 12 hours, LTG- treated group in which LTG （20mg/kg） was injected intraperitoneally iiranediately after HIBD and the mild hypothermia plus LTG group in which mild hypothermia was established for a duration of 12 hours after HIBD and LTG was injected immediately after HIBD. Twenty- four hours after operation, blood and brain samples were taken for serum neuron- specific enolase （NSE） test, immunohistochemical staining. Results The levels of serum NSE were significandy decreased and the numbers of NSE positive neurorts were markedly increased in the mild hypothermia plus LTG group as compared with mild hypothermia controls （ P 〈 0.01 ）. Conclusion Mild hypothermia plus Lamotrigine possess synergetic protective effect on hypoxic- ischemic brain damage.