摘要
用痘苗病毒作载体，成功构建了高效表达人癌胚抗原（ＣＥＡ）的ＣＥＡ－重组痘苗病毒，用１４３ＴＫ－细胞作为宿主细胞，表达的蛋白质只在细胞膜上能检测到，而胞质内和细胞上清液中均未能检测到，可见所表达的蛋白质保持了其原有特性，再次证实痘苗病毒是真核表达的有效载体。本结果为进一步研究ＣＥＡ－重组痘苗病毒能否激活机体的免疫系统杀伤表达ＣＥＡ的肿瘤提供了物质基础。
By using vaccinia virus as a vector, we have successfully established a CEA vaccinia virus recombinant capable of highly expressing CEA. After infecting 143TK cells with the recombinant, the expressed products could be detected by radio immunoassay technique only in cell membrane fragment but not in cell plasma and culture supernate. It further proved that vaccinia virus is an efficient vector for expressing eukaryotic protein and can maintain the characteristics of the protein. Based on this result, it is possible to study whether CEA vaccinia virus recombinant can activate immuno reactive system in vivo for combatting against tumors expressing CEA.
出处
《中山医科大学学报》
CSCD
1997年第1期9-12,共4页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
广东省卫生厅"九五"五个一工程项目资助
关键词
痘苗病毒
癌胚抗原
基因重组
肿瘤
基因治疗
vaccinia virus/genetics
carcinoembryonic antigen
gene expression
gene rearangment
