复方当归柴胡提取液对二乙基亚硝胺诱发大鼠肝癌前病变的抑制作用

INHIBITORY EFFECT OF COMPOSITE AQUOUS EXTRACT OF RADIX ANGELICAE SINENSIS AND RADIX BUPLEURUM ON DIETHYLNITROSAMINE INDUCED HEPATOCARCINOGENESIS IN RATS

为探索肝癌的化学预防，应用抑制二乙基亚硝胺（ＤＥＮ）致大鼠肝癌前病变肝细胞增生酶异常灶（ＧＧＴ灶）发生的短期实验模型，研究对肝炎有一定疗效的逍遥散加减为基础组成的复方当归、柴胡提取液（９５４合剂）对ＤＥＮ致肝癌作用的影响，并与已知抑制物甘草甜素（ＧＬ）相比较。结果显示９５４合剂和ＧＬ对ＤＥＮ诱发肝癌前病变ＧＧＴ灶的数量及大小均有明显抑制作用，与单给ＤＥＮ的对照组相比，差异非常显著（Ｐ＜０．０５～Ｐ＜０．００５），提示对启动和促进阶段均有抑制效果。对肝癌预防，尤其对暴露于肝炎病毒和化学致癌物的高危人群，有应用价值。

For investigating the chemoprophylaxis of liver cancer, the liquid extract composed mainly of Radix angelicae sinensis and Radix bupleurum (Complex 954) was tested. The animal model of diethylnitrosamine (DEN) induced precancerous lesion of liver with hyperplastic hepatocyte foci positive for gamma glutamyltranspeptidase (GGT) in male Sprague Dawley rats was used. Its effect was compared with that of Glycyrrhizin which had been confirmed as an inhibitor against the occurrence of GGT foci induced by aflatoxin B 1 (AFB 1) and DEN in rats. The results revealed that this extract exerts remarkable inhibition on the number and size of DEN induced GGT foci as compared with controls ( P<0.05￣P <0.005) and the effect is nearly the same as that of Glycyrrhizin, suggesting that this extract possesses the inhibitory effect both on the initiation and promotion stages during hepatocarcinogenesis. This inhibitor may be useful for the chemoprophylaxis of liver cancer, especially for the high risk population exposed to the hepatitis virus and chemical carcinogens.