PCNA和DNA含量估测腮腺混合瘤增殖活性

EVALUATION OF PCNA AND DNA CONTENTS IN STUDY OF CELLULAR PROLIFERATIVE ACTIVITY OF PAROTID GLAND MIXED TUMOR

为了评价增殖细胞核抗原（ＰＣＮＡ）表达和ＤＮＡ含量在研究腮腺混合瘤细胞增殖活性中的价值，对６０例良性无复发、复发和恶性腮腺混合瘤用ＰＣＮＡ免疫组化染色技术和流式细胞测量术作ＰＣＮＡ测定和ＤＮＡ含量测量。结果显示ＰＣＮＡ表达在各组间和高Ｓ期细胞比率（ＳＰＦ）在无复发和复发组间有显著差异；恶性混合瘤组与良性无复发组或复发组间有显著差异；ＰＣＮＡ表达与高ＳＰＦ呈显著性正相关关系。提示ＰＣＮＡ表达和ＤＮＡ含量均能反映良恶性混合瘤细胞增殖活性，对良恶性混合瘤的鉴别有重要价值；ＰＣＮＡ表达对良性混合瘤复发亦有较高估测价值。

For the purpose of evaluating the value of PCNA and DNA contents in cellular proliferative of parotid gland mixed tumor, the PCNA and DNA contents were analysed by immunohistochemical staining and flow cytometry for sixty cases of benign unrecurrent, recurrent and malignant parotid gland mixed tumor. The result showed that there were significant difference beween the groups; and there were no significant difference in incidence of aneuploid and high Sphase fraction (SPF) between unrecurrent and recurrent tumors and there were significant difference between benign and malignant tumors, and a linear positive correlation was observed between PCNA and high SPF. The results indicated that both PCNA and DNA contents can reflect the cellular proliferative activity and play an important role in distinguishing benign and malignant mixed tumors, that the PCNA is useful for judging the recurrent of benign mixed tumor.