两种寡糖对小鼠黑色素瘤实验性肝转移的抑制作用

Inhibitory effects of two oligosaccharides on murine melanoma expertenal metastasis

观察自行设计并化学合成的四糖及天然存在的酵母甘露聚糖对小鼠黑色素瘤实验性肝转移的影响。方法将０．５ｍｌ的１×１０６个Ｂ１６－ＭＢＫ黑色素瘤细胞与４ｍｇ四糖（四糖组）或４ｍｇ甘露聚糖（甘露聚糖组）体外３７℃预温３７分钟后，注入小鼠脾内。５５天后观察小鼠肝脏转移瘤的结节数、肝外转移情况及小鼠存活时间。结果对照组（Ｂ１６细胞＋磷酸盐酸缓冲液）６只小鼠中４只在接种Ｂ１６细胞后５５天内自然死亡，２只于第５５天处死。６只小鼠均在肝中有转移结节形成，瘤结节总数为２～３０个，最大瘤结节融合至整个肝脏，１只小鼠在注射局部也形成了肿瘤，３只伴有肺转移；四糖组６只小鼠中１只在第５０天死亡并在肝脏表面有３个瘤结节，最大直径为１０ｍｍ×８ｍｍ，其余５只均无肝转移，但有３只小鼠在注射局部形成了肿瘤；甘露聚糖组６只小鼠无提前死亡，仅１只有２个直径＜１ｍｍ的肝转移结节。四糖组及甘露糖聚组均无肝外转移发生，两组小鼠的肝脏湿重也明显低于对照组。结论四糖及甘露聚糖均能阻断小鼠黑色素瘤实验性肝转移，并抑制肝外转移。

Objective To observe the effects of achemically synthesized tetrose and anatural yeast mannan on mouse melanoma experimental liver metastasis.Methods After treated with 4mg tetrose(tetrose group)or 4mg mannan(mannan group)for 30 minutes at 37℃,0.5ml1×10 6B16 MBK melanoma cells were injected intraspleen.55 days later,melanoma metastasis nodes in the surfaces of the liver and othe rorgans as well as mouse survival time were observed.Reults Of 6 mice in control(B16cell+PBS),4 died naturally within55days,2 were dissected in the 55 th day.All of the 6 mice had metastases in the livers,the total number of the melanoma nodes on each liver surface ranged from 2 to 30,with the largest one fused to the whole liver.One mouse had aneoplasm in the remnant site of injection,3 had metastases in lungs,while of the 6 mice in tetrose group,one died on the 50thday of injection.In mannan group,all of the6mice survived and no metastasis was seen except the largest diameter of<1mm of 2 liver nodes in one mouse.Neither tetrose nor mannan group had metastasis of the liver,and the weights of liver in the two groups were significantly lower than in the control.Conclusion Both tetrose and mannan had the effects in blocking melanoma experimental liver metastasis,inhibiting transmigration of the liver,and prolonging the survival time of the mouse.