高效液相色谱-质谱/质谱联用法测定饮料中的人工合成色素

Determination of synthetical pigments in drinks by high performance liquid chromatogra-phy-mass spectrometry/mass spectrometry

目的:建立饮料中8种人工合成色素日落黄、柠檬黄、苋菜红、胭脂红、赤藓红、诱惑红、酸性靛蓝和亮蓝的定性、定量分析方法。方法:饮料样品经加热驱除二氧化碳或乙醇后直接过膜进样,以甲醇+乙酸铵(0.02 mol/L,pH=4.0)为流动相,梯度淋洗分离,电喷雾(ESI)离子化,选择反应监测方式(SRM)定性和定量。结果:样品处理简单,定性、定量准确,检测灵敏度高,回收率98.2%～101.6%,RSD0.3%～1.2%,相关系数0.9991～0.9999,检出限0.4～13μg/L。结论:该方法简单、准确、灵敏,适用于饮料中这8种人工合成色素的确证和定量分析。

Objectlve：To establish a method for determination of eight synthetical pigments in drinks. Methods：After being heated to dissipate carbon dioxide or ethanol, the samples were separated by reverse phase liquid chromatography using mobile phase of carbinol and acetic ammonium solution（0. 02 mol/L, pH = 4） mixture under gradient elution, confirmed and quantified by mass spectrum in the selected reaction monitoring （SRM） mode via positive electrospray ionization（ ESI ＋ ）. Results：Dealing with samples was very simple and the method had strong qualitative and quantitative ability. The curve of peak area with concentration was from 0. 1 to 100 mg/L with correlation coefficients from 0. 9991 to 0. 9999. The detection limit of this eight synthetical pigments was between 0. 4 and 13 μg/L. The recovery of the method was from 98.2% to 101.6%, and the relative standard deviation （RSD） was between 0. 3% and 1.2%. Conclusion：The method developed is sensitive, simple, accurate and can be used to confirm and quantify this eight synthetical pigments in drinks.