微量培养板不同保存时间对霍乱弧菌培养效果的观察

Observation on cultivation of Vibrio cholera of different conservation time with micro-plate cultivation technique

目的:通过用霍乱弧菌不定期地对冰箱内保存的微量培养板进行测试,观察不同保存时间微量板对细菌检测结果的影响,探讨微量板最佳保存时间。方法:采用霍乱弧菌常规培养法[1]与霍乱弧菌微量板培养法[2]平行实验,比较霍乱弧菌最小检出浓度。结果:用两株菌作为测试菌,每月1次共做了6批实验,微量板最长保存时间201 d。微量板培养法细菌最小检出浓度为100～102cfu/m l;常规培养法细菌最小检出浓度为101～102cfu/m l,两法对霍乱弧菌检测试验结果的敏感性较为一致。保存长达201 d的微量板未发现有培养基干裂、杂菌污染及颜色改变,并且也未影响检测结果。结论:微量培养板可以用于霍乱弧菌病原检测,结果证实微量板可在冰箱保存6个月以内使用。

Objective：To study the optimal conservation time of micro - plate by testing the Vibrio cholera conserved with micro - plates in a refrigerator to observe the affection on experimental results caused by different conservation time. Methods： Parallel experiments between routine V/br/o cultivation method and V/br/o micro - plate cultivation method were carried out to compare the minimum detection counts of Vibrio cholera. Results：Six tests were carried out in each month with two strains and as long as 201 - days conservation time was obtained with micro - plate method. Minimum detection counts was 10^0- 10^2 cfu/ml for micro - plate method and 101 - 102 cfu/ml for routine cultivation method. The sensitivity between two methods remained consistent. No crack, contamination and change of colors were found in 201 - days conserved micro - plates. Conclusion： Micro - plates stored for 6 months in a refrigerator can be used to detect Vibrio cholera.