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抗逆相关基因GmDREB导入农杆菌的研究 被引量：2

Study on Transformation of Stress Resistance Related Gene GmDREB into Agrobacterium tumefaciens
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摘要 利用改进的冻融法和电击法成功地将带有编码DREB转录因子GmDREB基因的prdGm-200双元载体分别导入根癌农杆菌EHA101、EHA105和LBA4404菌株中,同时探讨了影响冻融法和电击法转化效率的因素。结果表明:农杆菌细胞OD600约为0.6时冻融法和电击法的转化效率均较高。在利用冻融法转化农杆菌时,新制备的感受态细胞有利于提高转化效率。在进行电击法转化农杆菌时,电场强度和脉冲时间都对农杆菌的转化效率有影响。 The binary vector prdGM-200 with GmDREB gene coding for translation factor DREB was transformed into Agrobacterium tumefaciens strains EHA101, EHA105 and LBA4404 by using freeze-thaw method and electmporation respectively. The factors influencing transformation efficiencies through freezethaw and electmporation methods were studied. The results showed that higher transformation efficiency was observed when the value of OD600 reached 0.6. The new competent cell could increase transformation efficiency using freeze-thaw method. While transformation efficiency was influenced by electric field intensity and pulse time when electmporation method was applied.
作者 王萍 高世庆 郭永来 程宪国 王罡 马有志
机构地区 淮海工学院海洋学院 中国农业科学院作物科学研究所 长春龙洋大豆生物蛋白股份有限公司 中国农业科学院资源与农业区划研究所 天津大学农业与生物工程学院
出处 《吉林农业大学学报》 CAS CSCD 北大核心 2007年第2期148-151,共4页 Journal of Jilin Agricultural University
基金 教育部大豆生物学重点实验室资助项目(SB06A07) 国家植物转基因研究与产业化专项基金资助项目(JY03-A-18)
关键词 根癌农杆菌 GmDREB基因 转化 Agrobacterium tumefaciens GmDREB gene transformation
分类号 Q786 [生物学—分子生物学]
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参考文献12

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共引文献38

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二级引证文献7

吉林农业大学学报
2007年 第2期

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