突变型P53蛋白及DNA含量在宫颈癌动脉介入化疗前后的表达及意义

Significance of mutant P53 protein and DNA content in cervical cancer before and after intra-arterial infusion chemotherapy

目的检测突变型P53蛋白及DNA含量在宫颈癌新辅助动脉介入化疗前后的变化,探讨其与宫颈癌动脉介入化疗疗效的关系。方法取正常宫颈组、宫颈癌介入化疗前和化疗后4周各21例的宫颈组织标本,采用免疫组化方法进行突变型P53蛋白检测,用流式细胞术测定其DNA含量(DI)。结果21例宫颈癌患者中,动脉介入化疗前突变型P53蛋白检测阳性者5例,阳性率为23.81%;动脉介入化疗后突变型P53蛋白检测阳性者15例,阳性率为71.43%;两者之间有显著性差异(P<0.01);对照组无阳性表达。宫颈癌组介入化疗前和介入化疗后的DNA含量(DI)分别为1.40+0.33和1.21+0.16,有显著性差异(P<0.05),且均高于正常宫颈组1.09+0.10(P<0.01,P<0.05)。结论宫颈癌组织的突变型P53蛋白的表达在动脉介入化疗前后有显著性差异,动脉介入化疗可能诱导宫颈癌组织中突变型P53蛋白的表达。介入化疗能抑制宫颈癌DNA的复制,抑制肿瘤的增殖。

Objective To detect the change of mutant P53 protein and DNA content in cervical cancer before and after neoadjuvant intra-artefial infusion chemotherapy,to explore the relationship between them and efficiency of intra-arterial infusion chemotherapy. Methods Choose cervical squamous cell cancer tissues （21 eases） before and after intra-arterial infusion chemotherapy,normal cervical tissues （21 cases） as control group.The expression of mutant P53 protein was studied by means of immunohistochemistry,using flow eytometry to determined DNA content.Results Expression of mutant P53 protein was in 23.81%（5/21） and 71.43%（15/21） of cervical cancer before and after intra-arterial infusion chemotherapy among 21 cases,respectively.There was a significant difference between them（P〈0.01 ） ,None was seen of control group.The DI of cervical cancer tissues after intra-arterial infusion chemotherapy （1.2 ±0.16） was significantly lower than that before threatment（1.40±0.33）（P〈0.05）;The Dis were significant higher in cervical cancer tissues both before and after neoadjuvant intra-arterial infusion chemotherapy than that of normal cervix tissues（1.09±0.10）（P〈0.01 ,P〈0.05）.Conclusion Intra-arterial infusion chemotherapy may induce the expression of mutant P53 protein. Intra-arterial infusion chemotherapy can inhibit the DNA reproduction and the proliferation of the cancer.