siRNA对胰腺癌细胞c-Src表达的影响

Inhibition of the expression of c-Src gene in pancreatic carcinoma cells by small interfering

目的应用小干扰RNA（siRNA）干扰胰腺癌PANC—1细胞的c—Src表达，为后续试验做准备。方法将c—Src siRNA，经Lipofeetamine^TM2000脂质体转染胰腺癌PANC-1细胞后，用逆转录一聚合酶链反应（RT-PCR）和免疫印迹技术（Western blot）分别检测c—Src基因mRNA和c—Src蛋白表达的变化。结果转染后胰腺癌PANC—1细胞的c—Src的mRNA与蛋白表达明显减少。结论运用RNA干扰技术，可以有效地干扰胰腺癌PANC—1细胞c—Src的表达。

Objective To explore the effect of small interfering RNA （siRNA） on PANC-1 pancreatic carcinoma cells, the effective position in c-Src gene for siRNA. Methods siRNA targeting c-Src gene was transfected into PANC-1 cells by Lipofectamine^TM2000. The c-Src mRNA and c-Src protein were detected by reverse chain reaction （RT- PCR） and Western blot. Results The expression of c-Src gene in PANC-1 ceils could be effectively suppressed by siRNA. Conclusion The expression of c-Src in PANC-1 ceils can be decreased by RNA interference technique.