不同品系万寿菊花中叶黄素和叶黄素酯含量的测定

Determination of Contents of Lutein and Lutein Esters in Marigold Flowers(Tagetes erecta Linn.)

采用反相高效液相色谱法测定不同颜色万寿菊花(包括花瓣和花萼)中叶黄素和叶黄素酯的含量。通过与叶黄素标准品比较,并利用相同色谱条件下的色谱图确定万寿菊花中含有叶黄素和6种叶黄素二酯,即月桂酸-肉豆蔻酸酯、二肉豆蔻酸酯、肉豆蔻酸-棕榈酸酯、二棕榈酸酯、棕榈酸-硬脂酸酯及二硬脂酸酯。不同颜色万寿菊花中叶黄素和叶黄素酯的含量差别较大,黄绿色花色素含量为0.024%,橙色花色素含量高达1.148%(均以干花粉计),颜色越深,叶黄素酯含量越高。万寿菊花的色素含量与脂肪含量呈明显正相关(R2=0.974)。

Contents of lutein and lutein fatty acid esters in flower-heads （ petals and calyces） of different colors of marigold were evaluated by high performance liquid chromatography. A typical chromatographic profile of pigments was characterized by the simultaneous presence of lutein and lutein esters with standard samples of lutein. Six lutein esters from orange marigold flower, consisted of lutein laurate-myristate, dimyristate, myrlstate-palmitate, dipalmitate, palmitate-stearate, and distearate diesters, were confirmed. The total contents of pigments in dry marigold flower flour varied from 0. 024 % in greenish yellow flowers to 1. 148 % in deep orange flowers. Differences in petal color were well related to the pigment content. Furthermore, a positive correlation between pigment concentration and fat content in marigold flower was found （R^2 = 0. 974）.