细胞因子与抗肿瘤药物体外协同抑制宫颈癌细胞的敏感性试验

Study on the cell factor and the anti-tumor medicine with a sensitivity experiment of repress the Hela cell

目的探讨3种细胞因子单一、协同以及与化疗药物顺铂(DDP)的联合作用杀伤宫颈癌Hela细胞株的影响。方法应用四甲基偶氮唑盐比色法(MTT比色法)、检测与化疗药物DDP单独或联合应用组及药物作用的12 h与24 h的吸光度值(A),计算杀伤率。台盼蓝拒染计算杀伤率对照。结果单独应用3种细胞因子时,IFN-α(干扰素-α)、TNF-α(肿瘤坏死因子)对宫颈癌Hela有细胞毒作用,IL-2对宫颈癌Hela细胞株则细胞毒作用不明显。IFN-α、IL-2、TNF-α可以增强DDP的抑瘤作用,与DDP单独作用差异具有显著性(P<0.01)。IFN-α、IL-2(白细胞介素-2)、TNF-α联合作用具有协同作用,均与单一应用差异具有显著性(P<0.01)。细胞因子均可以增强DDP的抑瘤作用并与其作用的先后及时间具有依赖关系。结论MTT比色法为临床肿瘤化疗的药物敏感性检测提供了简便、快速的方法[1]。不同类型的肿瘤有着不同的药敏谱。IFN-α、TNF-α、IL-2与DDP联合具有协同作用,为宫颈癌患者化疗提供了理想的参考方案。

Objective To investigate the effects of three alone and in combination chemicals with DDP on Hela cell line. Methods Chemosensitivity of eels to doxorubicin was analysed using the MTT Assay 12 h and 24 h after using three cell genes alone and in combination chemicals with DDP chemotherapy drug,and compared using typanlan examined. Results There were significant diffenrnces effects on the Hela cell line in the inhibition rates of the between rhIFN-group, rhTNF-α group and negative control group. IL-2 had not strong activity of antitumor, rhTNF-α and rhIFN-α had a strong activity of antitumor in vitro and good dose-effect relationship compared with using DDP alone （ P 〈 0.01 ）. rhIFN-α,rhlL-2, rhTNF-α were significant diffenmces effects compared with using one alone group （P 〈 0.01 ）. rhIFN-α, rhlL-2, rhTNF-α with DDP had synergistic enhancement of antitumor activity in vitro and depend on time. Conclusions The MTT method is very rapid and simple to perform. Chemosensitivity spectra vary with tumor types. It can be applied to clinical drug sensitivity testing of human tumors and the results of this chemosensitivity will prvide the reference or ideal plan for the chemotherapy of cervical carcinoma patients.