中华真地鳖若虫肠道细菌的研究

Study on the intestinal bacteria of Eupolyphaga sinensis Walker

中华真地鳖是一种传统中药资源昆虫,从人工饲养种群的正常取食及蜕皮若虫肠道环境中分离、纯化,好氧和厌氧培养获得11个细菌菌株,对其形态、染色反应、培养性状和生理生化反应进行了系统研究。鉴定结果表明,上述11个细菌菌株依次分别属于:不动杆菌属(Acinetobacter)、柠檬酸杆菌属(Citobacter)、沙门菌属(Sal-monella)、芽胞乳酸杆菌属(Sporolacillus)、节杆菌属(Arthrobacter)、肠杆菌属(Enterobacter)、地杆菌属(Terrabacter)、放线杆菌属(Actinobacillus)、克雷伯菌属(Klebsiella)、沙雷菌属(Serratia)、预研菌属(Yokenella)。中华真地鳖正常取食和蜕皮若虫肠道环境细菌种类不同。分离未获得真菌和放线菌。

Eupolyphaga sinensis Walker was a kind of important traditional Chinese medicine resources insect, Intestinal bacteria of nympha of Eupolyphaga sinensis Walker,collected from natural and artificial population, had been isolated and identified, The identification results about 11 bacteria strains was that they belong to ：Acinetobacter, Citobacter, Salmonella, Sporolacillus , Arthrobacter , Enterobacter , Terrabacter , Actinobacillus , Klebsiella , Serratia , Yokenella respectively, The intestinal bacteria were different between eaten nympha and not of eaten Eupolyphaga sinensis Walker.