制备高活性α-干扰素实验条件的研究

THE RESERCH OF EXPERIMENTAL CONDITION ON PRODUCING HIGHER α IFN TITER

采用正交设计法对诱导α－ＩＦＮ产生的主要因素进行研究。结果表明，人白细胞数对α－ＩＦＮ活性影响作用最大，其次为ｐＨ，血浆浓度，ＮＤＶ－Ｆ血凝单位。并获得制备高活性α－ＩＦＮ最适实验条件是：人白细胞数３０×１０９Ｌ－１，血浆浓度２％，ＤＮＶ－Ｆ血凝单位１２５６０，ｐＨ７．４，为提高α－ＩＦＮ的产量提供了可靠参数。

This article reported major factors of inducing α IFN by a reasonable orthogonal design.The results showed that amount of HWBC was the most principal influence on α INF titer.The next was PH,plasma concentration and NDV F titer.While have got optimal experimental condition of producing higher α IFN titer.They were amount of HWBC 30×10 9 L -1 ,plasma concentration 2%,NDV F titer 1∶2 560 and PH7.4.The authors provided relible parameter for raising output of α IFN.