细胞因子对肾小球系膜细胞增殖的影响

EFFECT OF CYTOKINES ON PROLIFERATION OF HUMAN MESANGIAL CELLS

应用３Ｈ－ＴｄＲ掺入法和ＭＴＴ比色法，观察了人重组ＩＬ－１β、ＩＬ－６和ＴＮＦα对体外培养的成人肾小球系膜细胞增殖的影响。结果表明，在无血清培养条件下，ＨｒＩＬ－１β、ＨｒＩＬ－６和ＨｒＴＮＦα在一个较大的浓度范围内，均不能刺激系膜细胞的增殖；而有少量胎牛血清存在时，ＨｒＩＬ－６和ＨｒＴＮＦα可以促进系膜细胞增殖，但ＨｒＩＬ－１β无此作用。另外，在实验中还发现，雷公藤多甙（ＴⅡ）对系膜细胞生长具有抑制作用。

Both IL 6 and TNFα stimulated human mesangial cells (HMC) to proliferate,as measured by 3H TdR incorporation and MTT uptake.But it wasnt able to induce HMC to proliferate with IL 1β either in serum free medium or in the presence of 5% fetal calf serum.It suggested that some cytokines might be play an important role in mesangial proliferative glomerulonephritis.In addition,HMC proliferation could be inhibited by a Chinese drug T Ⅱ