摘要
本文报道应用人外周血淋巴细胞在离体条件下,用低剂量^(60)Coγ线照射后与M期CHO细胞融合产生G_1-PCC,计数其畸变产额,得到线性剂量效应刻度曲线,Y=0.26+0.028.Dr=0.99,并与常规细胞遗传学方法作了比较,结果表明G_1-PCC方法比常规遗传学方法快速、灵敏。对其作为生物剂量计尚存在的问题也进行了讨论。
The human blood was irradiated in vitro by 0-100 10^(-2)Gy. Then thelymphocytes were separated from the whole blood. The mitotic CHO cells fused withlymphocytes. This technique can be used to visualize damage to interphase chromosomeaberrations after irradiation. The frequency of breaks produced in G_1 phase of the ly-mphocytes is scord. The dose-response curve for chromosome fragments was found tobe linear Y=0. 26+0. 028. D, r=0. 99. The method was compared with conventionalcytogenetic. The results presented that the G_1-PCC technique is more rapidly andsensitively. But the G_1-PCC used technique of biological dosimetry has some questionswhich is discussed.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
北大核心
1990年第2期107-110,共4页
Journal of Radiation Research and Radiation Processing
关键词
辐射
染色体
淋巴细胞
人体
畸变
Premature condensation chromosome
Aberration
Break
guestions
