东亚飞蝗肠道细菌的研究

Study on the intestinal bacteria in L.migratoria manilensis

目的从微生态学角度研究东亚飞蝗的营养生理活动。方法从东亚飞蝗自然种群的雌、雄成虫肠道环境中分离、纯化、培养,获得细菌16个属的菌株,对菌体形态、染色反应、培养性状、生理生化反应进行系统研究。结果上述16个细菌菌株分别属于沙雷菌属(Serratia)、短状杆菌属(Brachybacterium)、预研菌属(Yokenella)、肠杆菌属(Penterobacter)、微杆菌属(Microbacterium)、柠檬酸杆菌属(Citrobacter)、类芽胞杆菌属(Paenibacillus)、沙门菌属(Salmonella)、棍状杆菌属(Clavibacter)、放线杆菌属(Actinobacillus)、米勒菌属(Moelleralla)、不动杆菌属(Acinetobacter)、埃希菌属(Escherichia)、葡萄球菌属(Staphylococcus)、克吕沃尔菌属(Kluyvera)、克雷伯菌属(Kleb-siella)。结论东亚飞蝗雌性成虫肠道环境细菌种类为12个,雄性为10个,其中6个菌株相同;数量之间存在明显差异。

L. migratoria manilensis Meyer is a very important species in ecosystem, and a species of very important agricultural pest insect, or a important resource of protein from insects. Sixteen bacteria strains were isolated from the intestinal of the adults（ ♀/♂ ）that were bred in lab. And the bacterial modality, dyeing reaction, cultural character and physiological and biochemical reactions were studied. The result of identification were that the sixteen bacterial strains belonged to Serratia, Brachybacterium, Yokenella , Penterobacter , Microbacterium, Citrobacter , Paenibacillus , Salmonella, Clavibacter ,Actinobacillus , Moelleralla ,Acinetobacter , Escherichia , Staphylococcus, Kluyvera , Klebsiella. The number of bacteria strain from female was 12 and male was 10 and 6 of them were the same;tile quantitiy of intestinal bacteria was different between the adults（ ♀ ／ ♂ ）.